Journal of General Virology, Vol 78, 2447-2451, Copyright © 1997 by Society for General Microbiology

ARTICLES

Influenza virus NS1 protein interacts with viral transcription- replication complexes in vivo

RM Marion, T Zurcher, S de la Luna and J Ortin
Centro Nacional de Biotecnologia (CSIC), Campus de Cantoblanco, Madrid, Spain.

The interaction of influenza virus NS1 protein with other viral products in the infected cell was analysed by co-immunoprecipitation studies. The three subunits of the polymerase and the nucleoprotein, but not M1 protein, were co-immunoprecipitated by NS1-specific serum but not when control serum was used. Such co-immunoprecipitation was not sensitive to RNase treatment of the immunoprecipitates. Co- immunoprecipitation was also obtained when the viral transcription- replication system was reconstituted in vivo by transfection of cDNAs and model vRNA template into vaccinia virus-T7-infected cells. Analysis of the RNA pulled-down in the NS1-specific precipitates indicated the presence of both vRNA and mRNA. These results are discussed in the context of the phenotype of virus temperature-sensitive mutants affected in the NS1 gene.

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