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J Gen Virol 78 (1997), 2587-2590
© 1997 Society for General Microbiology
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Journal of General Virology, Vol 78, 2587-2590, Copyright © 1997 by Society for General Microbiology

ARTICLES

Inhibition of Moloney murine leukaemia virus by a retroviral vector, LNL6, carrying ribozymes targeted to the 5' non-coding sequence

P Lowenstein and G Symonds
Johnson & Johnson Research Laboratories, Sydney, NSW, Australia.

The packaging region psi is the RNA sequence which directs the inclusion of genomic viral RNA into virions. As such it represents an apparently accessible site for ribozyme action. Ribozymes directed against the psi site of Moloney murine leukaemia virus (MoMLV) were delivered to target cells using a related retroviral vector, LNL6. LNL6 is a vector predominantly derived from MoMLV and, like all retroviruses, requires the psi region to be packaged. By exploiting the heterogeneity between nucleotide sequences of the MoMLV target and LNL6 vector in the psi packaging region, two ribozymes were designed and shown to selectively cleave the target but not the vector sequence. Clonally derived cell lines expressing ribozymes showed inhibition of virus replication. These results show the utility of catalytic RNA as specific antiviral agents.




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Copyright © 1997 by the Society for General Microbiology.