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J Gen Virol 78 (1997), 2747-2750
© 1997 Society for General Microbiology
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Journal of General Virology, Vol 78, 2747-2750, Copyright © 1997 by Society for General Microbiology

ARTICLES

Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: a case against extrahepatic replication

T Laskus, M Radkowski, LF Wang, J Cianciara, H Vargas and J Rakela
Division of Transplantation Medicine, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, PA 15213, USA.

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.


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