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J Gen Virol 78 (1997), 3167-3170
© 1997 Society for General Microbiology

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Journal of General Virology, Vol 78, 3167-3170, Copyright © 1997 by Society for General Microbiology


ARTICLES

Isolation and characterization of tubular structures of cowpea mosaic virus

DT Kasteel, J Wellink, RW Goldbach and JW van Lent
Department of Virology, Agricultural University, Wageningen, The Netherlands.

Tubular structures involved in the cell-to-cell movement of cowpea mosaic virus (CPMV) were partially purified from infected cowpea protoplasts to identify the structural components. A relatively pure fraction could be obtained by differential centrifugation and this was analysed by PAGE and immunoblotting. Besides the movement protein (MP) and capsid proteins (CP) of CPMV, no other major infection-specific proteins could be detected, suggesting that host proteins are not a major structural component of the movement tubule.


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