Journal of General Virology, Vol 78, 627-635, Copyright © 1997 by Society for General Microbiology

ARTICLES

Analysis of vif-defective human immunodeficiency virus type 1 (HIV-1) virions synthesized in 'non-permissive' T lymphoid cells stably infected with selectable HIV-1

C Ochsenbauer, T Wilk and V Bosch
Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

In order to facilitate analyses of the molecular function of the human immunodeficiency virus type 1 (HIV-1) Vif protein, we have developed a cell culture model-system which allows permanent production of genotypically and phenotypically vif-defective HIV-1 virions in 'non- permissive' H9 cells. Using recombinant, replication-competent HIV-1 proviruses coding for a selectable marker gene (gpt) instead of nef, two stably infected H9 subclones named M2 (vif-mutant) and WX (wild- type), respectively, were generated. Virions released from cell line M2- -displaying the expected vif-defective phenotype--are produced permanently, and in an at least 50 times higher amount than virus particles from acutely vif-negative HIV-1-infected H9 cells. Analysis of viral protein composition and the electron-microscopic morphology of vif-mutant virions did not reveal any detectable differences in comparison to wild-type virions.

This article has been cited by other articles:

				A. Kataropoulou, C. Bovolenta, A. Belfiore, S. Trabatti, A. Garbelli, S. Porcellini, R. Lupo, and G. Maga Mutational analysis of the HIV-1 auxiliary protein Vif identifies independent domains important for the physical and functional interaction with HIV-1 reverse transcriptase Nucleic Acids Res., June 1, 2009; 37(11): 3660 - 3669. [Abstract] [Full Text] [PDF]

				B. Liu, X. Yu, K. Luo, Y. Yu, and X.-F. Yu Influence of Primate Lentiviral Vif and Proteasome Inhibitors on Human Immunodeficiency Virus Type 1 Virion Packaging of APOBEC3G J. Virol., February 15, 2004; 78(4): 2072 - 2081. [Abstract] [Full Text] [PDF]

				S. Kao, M. A. Khan, E. Miyagi, R. Plishka, A. Buckler-White, and K. Strebel The Human Immunodeficiency Virus Type 1 Vif Protein Reduces Intracellular Expression and Inhibits Packaging of APOBEC3G (CEM15), a Cellular Inhibitor of Virus Infectivity J. Virol., November 1, 2003; 77(21): 11398 - 11407. [Abstract] [Full Text] [PDF]

				N. C. Gaddis, E. Chertova, A. M. Sheehy, L. E. Henderson, and M. H. Malim Comprehensive Investigation of the Molecular Defect in vif-Deficient Human Immunodeficiency Virus Type 1 Virions J. Virol., May 15, 2003; 77(10): 5810 - 5820. [Abstract] [Full Text] [PDF]

				S. Kao, H. Akari, M. A. Khan, M. Dettenhofer, X.-F. Yu, and K. Strebel Human Immunodeficiency Virus Type 1 Vif Is Efficiently Packaged into Virions during Productive but Not Chronic Infection J. Virol., December 20, 2002; 77(2): 1131 - 1140. [Abstract] [Full Text] [PDF]

				N. Madani, R. Millette, E. J. Platt, M. Marin, S. L. Kozak, D. B. Bloch, and D. Kabat Implication of the Lymphocyte-Specific Nuclear Body Protein Sp140 in an Innate Response to Human Immunodeficiency Virus Type 1 J. Virol., October 2, 2002; 76(21): 11133 - 11138. [Abstract] [Full Text] [PDF]

				M. A. Khan, H. Akari, S. Kao, C. Aberham, D. Davis, A. Buckler-White, and K. Strebel Intravirion Processing of the Human Immunodeficiency Virus Type 1 Vif Protein by the Viral Protease May Be Correlated with Vif Function J. Virol., August 12, 2002; 76(18): 9112 - 9123. [Abstract] [Full Text] [PDF]

				M. Bardy, B. Gay, S. Pebernard, N. Chazal, M. Courcoul, R. Vigne, E. Decroly, and P. Boulanger Interaction of human immunodeficiency virus type 1 Vif with Gag and Gag-Pol precursors: co-encapsidation and interference with viral protease-mediated Gag processing J. Gen. Virol., November 1, 2001; 82(11): 2719 - 2733. [Abstract] [Full Text] [PDF]

				M. A. Khan, C. Aberham, S. Kao, H. Akari, R. Gorelick, S. Bour, and K. Strebel Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA J. Virol., August 15, 2001; 75(16): 7252 - 7265. [Abstract] [Full Text] [PDF]

				T. Henzler, A. Harmache, H. Herrmann, H. Spring, M. Suzan, G. Audoly, T. Panek, and V. Bosch Fully functional, naturally occurring and C-terminally truncated variant human immunodeficiency virus (HIV) Vif does not bind to HIV Gag but influences intermediate filament structure J. Gen. Virol., March 1, 2001; 82(3): 561 - 573. [Abstract] [Full Text]

				A. Öhagen and D. Gabuzda Role of Vif in Stability of the Human Immunodeficiency Virus Type 1 Core J. Virol., December 1, 2000; 74(23): 11055 - 11066. [Abstract] [Full Text]

				M. Dettenhofer, S. Cen, B. A. Carlson, L. Kleiman, and X.-F. Yu Association of Human Immunodeficiency Virus Type 1 Vif with RNA and Its Role in Reverse Transcription J. Virol., October 1, 2000; 74(19): 8938 - 8945. [Abstract] [Full Text]

				N. Madani and D. Kabat Cellular and Viral Specificities of Human Immunodeficiency Virus Type 1 Vif Protein J. Virol., July 1, 2000; 74(13): 5982 - 5987. [Abstract] [Full Text]

				J. H. M. Simon, E. A. Carpenter, R. A. M. Fouchier, and M. H. Malim Vif and the p55Gag Polyprotein of Human Immunodeficiency Virus Type 1 Are Present in Colocalizing Membrane-Free Cytoplasmic Complexes J. Virol., April 1, 1999; 73(4): 2667 - 2674. [Abstract] [Full Text]

				J. H. M. Simon, A. M. Sheehy, E. A. Carpenter, R. A. M. Fouchier, and M. H. Malim Mutational Analysis of the Human Immunodeficiency Virus Type 1 Vif Protein J. Virol., April 1, 1999; 73(4): 2675 - 2681. [Abstract] [Full Text]

				M. Dettenhofer and X.-F. Yu Highly Purified Human Immunodeficiency Virus Type 1 Reveals a Virtual Absence of Vif in Virions J. Virol., February 1, 1999; 73(2): 1460 - 1467. [Abstract] [Full Text]