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J Gen Virol 78 (1997), 655-658
© 1997 Society for General Microbiology
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Journal of General Virology, Vol 78, 655-658, Copyright © 1997 by Society for General Microbiology

ARTICLES

Detection and characterization of human papillomavirus type 45 DNA in the cervical carcinoma cell line MS751

J Geisbill, U Osmers and M Durst
Angewandte Tumorvirologie, Deutches Krebsforschungszentrum, Heidelberg, Germany.

The cervical carcinoma-derived cell line MS751 was examined for human papillomavirus (HPV) DNA and RNA. A genomic fragment containing both viral and cellular sequences was cloned. Sequence analysis showed that MS751 cells contain a partially deleted HPV-45 genome integrated at a single chromosomal site. HPV sequences from the E6-E7 region are expressed as poly(A)+ RNA.


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