Journal of General Virology, Vol 78, 975-978, Copyright © 1997 by Society for General Microbiology

ARTICLES

PrP genotypes and experimental scrapie in orally inoculated Suffolk sheep in the United States

KI O'Rourke, GR Holyoak, WW Clark, JR Mickelson, S Wang, RP Melco, TE Besser and WC Foote
United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman 99164-7030, USA. korourke@vetmed.wsu.edu

One-hundred and three Unites States Suffolk sheep were inoculated orally with a scrapie agent preparation and monitored for clinical disease and histopathological lesions characteristic of scrapie. A retrospective study of the polymorphisms at codon 171 of the prion protein (PrP) gene was performed on these sheep. All 63 sheep that developed scrapie during the observation period were homozygous for the glutamine 171 (171-QQ) PrP allele. Twelve 171-QQ sheep failed to develop disease. All 5 sheep homozygous for arginine (171-RR) and all 23 heterozygous (171-QR) sheep remained free of scrapie.

This article has been cited by other articles:

				J. T. McKay, T. A. Brigner, B. E. Caplin, K. S. McCurdy, and R. L. Forde A real-time polymerase chain reaction assay to detect single nucleotide polymorphisms at codon 171 in the prion gene for the genotyping of scrapie susceptibility in sheep J Vet Diagn Invest, March 1, 2008; 20(2): 209 - 212. [Abstract] [Full Text] [PDF]

				A. N. Hamir, R. A. Kunkle, J. A. Richt, J. M. Miller, and J. J. Greenlee Experimental Transmission of US Scrapie Agent by Nasal, Peritoneal, and Conjunctival Routes to Genetically Susceptible Sheep Vet. Pathol., January 1, 2008; 45(1): 7 - 11. [Abstract] [Full Text] [PDF]

				L. Austbo, A. Espenes, I. Olsaker, C. McL. Press, and G. Skretting Increased PrP mRNA expression in lymphoid follicles of the ileal Peyer's patch of sheep experimentally exposed to the scrapie agent J. Gen. Virol., July 1, 2007; 88(7): 2083 - 2090. [Abstract] [Full Text] [PDF]

				M. L. Johnson, J. M. Evoniuk, C. L. Stoltenow, K. I. O'Rourke, and D. A. Redmer Development of an assay to determine single nucleotide polymorphisms in the prion gene for the genetic diagnosis of relative susceptibility to classical scrapie in sheep J Vet Diagn Invest, January 1, 2007; 19(1): 73 - 77. [Abstract] [Full Text] [PDF]

				W. Goldmann, F. Houston, P. Stewart, M. Perucchini, J. Foster, and N. Hunter Ovine prion protein variant A136R154L168Q171 increases resistance to experimental challenge with bovine spongiform encephalopathy agent J. Gen. Virol., December 1, 2006; 87(12): 3741 - 3745. [Abstract] [Full Text] [PDF]

				A. N. Hamir, R. A. Kunkle, R. C. Cutlip, J. M. Miller, E. S. Williams, and J. A. Richt Transmission of chronic wasting disease of mule deer to Suffolk sheep following intracerebral inoculation J Vet Diagn Invest, November 1, 2006; 18(6): 558 - 565. [Abstract] [Full Text] [PDF]

				L. Alvarez, J. J. Arranz, and F. S. Primitivo Identification of a new leucine haplotype (ALQ) at codon 154 in the ovine prion protein gene in Spanish sheep J Anim Sci, February 1, 2006; 84(2): 259 - 265. [Abstract] [Full Text] [PDF]

				E. S. Williams Chronic Wasting Disease Vet. Pathol., September 1, 2005; 42(5): 530 - 549. [Abstract] [Full Text] [PDF]

				J. E. Jewell, M. M. Conner, L. L. Wolfe, M. W. Miller, and E. S. Williams Low frequency of PrP genotype 225SF among free-ranging mule deer (Odocoileus hemionus) with chronic wasting disease J. Gen. Virol., August 1, 2005; 86(8): 2127 - 2134. [Abstract] [Full Text] [PDF]

				C. Diaz, Z. G. Vitezica, R. Rupp, O. Andreoletti, and J. M. Elsen Polygenic variation and transmission factors involved in the resistance/susceptibility to scrapie in a Romanov flock J. Gen. Virol., March 1, 2005; 86(3): 849 - 857. [Abstract] [Full Text] [PDF]

				T. Moum, I. Olsaker, P. Hopp, T. Moldal, M. Valheim, T. Moum, and S. L. Benestad Polymorphisms at codons 141 and 154 in the ovine prion protein gene are associated with scrapie Nor98 cases J. Gen. Virol., January 1, 2005; 86(1): 231 - 235. [Abstract] [Full Text] [PDF]

				P A Caplazi, K I O'Rourke, and T V Baszler Resistance to scrapie in PrP ARR/ARQ heterozygous sheep is not caused by preferential allelic use J. Clin. Pathol., June 1, 2004; 57(6): 647 - 650. [Abstract] [Full Text] [PDF]

				K. I. O'Rourke, T. R. Spraker, L. K. Hamburg, T. E. Besser, K. A. Brayton, and D. P. Knowles Polymorphisms in the prion precursor functional gene but not the pseudogene are associated with susceptibility to chronic wasting disease in white-tailed deer J. Gen. Virol., May 1, 2004; 85(5): 1339 - 1346. [Abstract] [Full Text] [PDF]

				C. Billinis, V. Psychas, L. Leontides, V. Spyrou, S. Argyroudis, I. Vlemmas, S. Leontides, T. Sklaviadis, and O. Papadopoulos Prion protein gene polymorphisms in healthy and scrapie-affected sheep in Greece J. Gen. Virol., February 1, 2004; 85(2): 547 - 554. [Abstract] [Full Text] [PDF]

				K. I. O'Rourke, J. V. Duncan, J. R. Logan, A. K. Anderson, D. K. Norden, E. S. Williams, B. A. Combs, R. H. Stobart, G. E. Moss, and D. L. Sutton Active Surveillance for Scrapie by Third Eyelid Biopsy and Genetic Susceptibility Testing of Flocks of Sheep in Wyoming Clin. Vaccine Immunol., September 1, 2002; 9(5): 966 - 971. [Abstract] [Full Text] [PDF]

				W. Tuo, K. I. O'Rourke, D. Zhuang, W. P. Cheevers, T. R. Spraker, and D. P. Knowles Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep PNAS, April 10, 2002; (2002) 72071199. [Abstract] [Full Text] [PDF]

				R. Heggebo, C. McL. Press, G. Gunnes, L. Gonzalez, and M. Jeffrey Distribution and accumulation of PrP in gut-associated and peripheral lymphoid tissue of scrapie-affected Suffolk sheep J. Gen. Virol., February 1, 2002; 83(2): 479 - 489. [Abstract] [Full Text] [PDF]

				N. E. COCKETT, T. L. SHAY, and M. SMIT Analysis of the sheep genome Physiol Genomics, December 21, 2001; 7(2): 69 - 78. [Abstract] [Full Text] [PDF]

				K. I. O'Rourke, T. V. Baszler, T. E. Besser, J. M. Miller, R. C. Cutlip, G. A. H. Wells, S. J. Ryder, S. M. Parish, A. N. Hamir, N. E. Cockett, et al. Preclinical Diagnosis of Scrapie by Immunohistochemistry of Third Eyelid Lymphoid Tissue J. Clin. Microbiol., September 1, 2000; 38(9): 3254 - 3259. [Abstract] [Full Text]

				R. Heggebø, C. McL. Press, G. Gunnes, K. Inge Lie, M. A. Tranulis, M. Ulvund, M. H. Groschup, and T. Landsverk Distribution of prion protein in the ileal Peyer's patch of scrapie-free lambs and lambs naturally and experimentally exposed to the scrapie agent J. Gen. Virol., September 1, 2000; 81(9): 2327 - 2337. [Abstract] [Full Text]

				S. B. Prusiner Prions PNAS, November 10, 1998; 95(23): 13363 - 13383. [Abstract] [Full Text] [PDF]

				K. Kaneko, L. Zulianello, M. Scott, C. M. Cooper, A. C. Wallace, T. L. James, F. E. Cohen, and S. B. Prusiner Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation PNAS, September 16, 1997; 94(19): 10069 - 10074. [Abstract] [Full Text] [PDF]

				T. L. James, H. Liu, N. B. Ulyanov, S. Farr-Jones, H. Zhang, D. G. Donne, K. Kaneko, D. Groth, I. Mehlhorn, S. B. Prusiner, et al. Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform PNAS, September 16, 1997; 94(19): 10086 - 10091. [Abstract] [Full Text] [PDF]

				W. Tuo, D. Zhuang, D. P. Knowles, W. P. Cheevers, M.-S. Sy, and K. I. O'Rourke PrP-C and PrP-Sc at the Fetal-Maternal Interface J. Biol. Chem., May 18, 2001; 276(21): 18229 - 18234. [Abstract] [Full Text] [PDF]

				W. Tuo, K. I. O'Rourke, D. Zhuang, W. P. Cheevers, T. R. Spraker, and D. P. Knowles Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep PNAS, April 30, 2002; 99(9): 6310 - 6315. [Abstract] [Full Text] [PDF]