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Journal of General Virology, Vol 78, 1049-1057, Copyright © 1997 by Society for General Microbiology
ARTICLES |
CW Mandl, M Ecker, H Holzmann, C Kunz and FX Heinz
Institute of Virology, University of Vienna, Austria.
Infectious cDNA clones of two strains of tick-borne encephalitis (TBE) virus, i.e. European subtype prototypic strain Neudoerfl and the closely related but more virulent strain Hypr, were constructed. The recombinant constructs consisted of cDNAs stably inserted into the bacterial plasmid pBR322 under the control of T7 promoter elements. The genome of TBE virus strain Neudoerfl was successfully cloned, both as a full-length cDNA and as two partial cDNAs. In the case of strain Hypr, the genome is represented by two cDNA clones corresponding to the 5'- and 3'-terminal halves of the genome. Highly infectious RNAs can be produced from the full-length cDNA clone or from the partial clones ligated in vitro to form full-length cDNA templates prior to T7 transcription. The biological properties of the recombinant progeny viruses, including virulence characteristics, were indistinguishable from the corresponding parent virus strains. Thus, the described infectious cDNA clones represent a useful and reliable experimental system for the specific mutagenesis of TBE virus.
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C. W. Mandl, H. Holzmann, T. Meixner, S. Rauscher, P. F. Stadler, S. L. Allison, and F. X. Heinz Spontaneous and Engineered Deletions in the 3' Noncoding Region of Tick-Borne Encephalitis Virus: Construction of Highly Attenuated Mutants of a Flavivirus J. Virol., March 1, 1998; 72(3): 2132 - 2140. [Abstract] [Full Text] [PDF]
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M. Yanagi, R. H. Purcell, S. U. Emerson, and J. Bukh Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee PNAS, August 5, 1997; 94(16): 8738 - 8743. [Abstract] [Full Text] [PDF]
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