J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 78 (1997), 1213-1217
© 1997 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Joelson, T.
Right arrow Articles by Morris, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Joelson, T.
Right arrow Articles by Morris, T. J.
Agricola
Right arrow Articles by Joelson, T.
Right arrow Articles by Morris, T. J.

Journal of General Virology, Vol 78, 1213-1217, Copyright © 1997 by Society for General Microbiology

ARTICLES

Presentation of a foreign peptide on the surface of tomato bushy stunt virus

T Joelson, L Akerblom, P Oxelfelt, B Strandberg, K Tomenius and TJ Morris
Department of Molecular Biology, Uppsala University, Sweden. thorleif.joelson@bmc.uu.se

A 13-amino-acid peptide derived from the V3 loop of human immunodeficiency virus (HIV-1) glycoprotein 120 (gp120) was attached as a C-terminal gene fusion to the coat protein of tomato bushy stunt virus (TBSV). The architecture of this plant virus permitted external display of the foreign sequence 180 times on the surface of the chimaeric virus particle. The chimaera replicated to a level similar to wild-type TBSV and the foreign sequence was retained through six sequential passages in plants. The HIV epitope was detected on the surface of the virus capsid by a V3-specific monoclonal antibody and by human sera from HIV-1-positive patients, demonstrating the potential of using plant-derived chimaeric particles for diagnostic purposes. Chimaeric virus also induced a specific immune response to the foreign HIV epitope when injected into NMRI mice.


This article has been cited by other articles:


Home page
 CVIHome page
Y. J. P. Ramirez, E. Tasciotti, A. Gutierrez-Ortega, A. J. D. Torres, M. T. O. Flores, M. Giacca, and M. A. G. Lim
Fruit-Specific Expression of the Human Immunodeficiency Virus Type 1 Tat Gene in Tomato Plants and Its Immunogenic Potential in Mice
Clin. Vaccine Immunol., June 1, 2007; 14(6): 685 - 692.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. R. Fernandez-Fernandez, J. L. Martinez-Torrecuadrada, F. Roncal, E. Dominguez, and J. A. Garcia
Identification of Immunogenic Hot Spots within Plum Pox Potyvirus Capsid Protein for Efficient Antigen Presentation
J. Virol., November 13, 2002; 76(24): 12646 - 12653.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
H. BELANGER, N. FLEYSH, S. COX, G. BARTMAN, D. DEKA, M. TRUDEL, H. KOPROWSKI, and V. YUSIBOV
Human respiratory syncytial virus vaccine antigen produced in plants
FASEB J, November 1, 2000; 14(14): 2323 - 2328.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Modelska, B. Dietzschold, N. Sleysh, Z. F. Fu, K. Steplewski, D. C. Hooper, H. Koprowski, and V. Yusibov
Immunization against rabies with plant-derived antigen
PNAS, March 3, 1998; 95(5): 2481 - 2485.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1997 by the Society for General Microbiology.