| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of General Virology, Vol 78, 1551-1557, Copyright © 1997 by Society for General Microbiology
ARTICLES |
CJ Tarbatt, GM Glasgow, DA Mooney, BJ Sheahan and GJ Atkins
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin, Ireland.
The nonstructural region of the genome of the avirulent A7 strain of Semliki Forest virus (SFV) has been sequenced, so that the complete nucleotide sequence is available. Compared to the virulent SFV4 strain (produced from the infectious clone pSP6-SFV4), A7 contains 226 nucleotide changes in the translated region, which result in 47 amino acid changes. The 5' nontranslated region has two nucleotide changes, and the 3' nontranslated region is longer in A7 than SFV4, and contains divergent and repeated sequences. Chimeras containing SFV4 and A7 sequences and an infectious clone of A7, pSP6-CA7, were constructed. The virulence of these was tested by intraperitoneal and intranasal infection of adult BALB/c mice. It was shown that determination of the avirulent phenotype of A7 was polygenic, and required the additive effect of sequences from both the structural and non-structural regions of the SFV genome.
This article has been cited by other articles:
|
|
 |
|
  S. A. Deuber and J. Pavlovic Virulence of a mouse-adapted Semliki Forest virus strain is associated with reduced susceptibility to interferon J. Gen. Virol., July 1, 2007; 88(7): 1952 - 1959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhou, H. Ushijima, and T. K. Frey Genomic analysis of diverse rubella virus genotypes J. Gen. Virol., March 1, 2007; 88(3): 932 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Galbraith, B. J. Sheahan, and G. J. Atkins Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence. J. Gen. Virol., April 1, 2006; 87(Pt 4): 937 - 947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. P. GREENE, S. PAESSLER, M. ANISHCHENKO, D. R. SMITH, A. C. BRAULT, I. FROLOV, and S. C. WEAVER VENEZUELAN EQUINE ENCEPHALITIS VIRUS IN THE GUINEA PIG MODEL: EVIDENCE FOR EPIZOOTIC VIRULENCE DETERMINANTS OUTSIDE THE E2 ENVELOPE GLYCOPROTEIN GENE Am J Trop Med Hyg, March 1, 2005; 72(3): 330 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tuittila and A. E. Hinkkanen Amino acid mutations in the replicase protein nsP3 of Semliki Forest virus cumulatively affect neurovirulence J. Gen. Virol., June 1, 2003; 84(6): 1525 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Fazakerley, A. Boyd, M. L. Mikkola, and L. Kaariainen A Single Amino Acid Change in the Nuclear Localization Sequence of the nsP2 Protein Affects the Neurovirulence of Semliki Forest Virus J. Virol., January 1, 2002; 76(1): 392 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Atkins, B. J. Sheahan, and P. Liljeström The molecular pathogenesis of Semliki Forest virus: a model virus made useful? J. Gen. Virol., September 1, 1999; 80(9): 2287 - 2297. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
