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J Gen Virol 78 (1997), 1779-1783
© 1997 Society for General Microbiology
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Journal of General Virology, Vol 78, 1779-1783, Copyright © 1997 by Society for General Microbiology

ARTICLES

Detection of potato mop-top virus capsid readthrough protein in virus particles

GH Cowan, L Torrance and B Reavy
Scottish Crop Research Institute, Invergowrie, Dundee, UK.

Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.


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