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J Gen Virol 78 (1997), 1829-1832
© 1997 Society for General Microbiology
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Journal of General Virology, Vol 78, 1829-1832, Copyright © 1997 by Society for General Microbiology

ARTICLES

Immunogenic, non-infectious polio subviral particles synthesized in Saccharomyces cerevisiae

B Rombaut and JP Jore
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Brussels, Belgium. brombaut@mich.vub.ac.be

Polioviral genes coding for P1, the precursor for the structural proteins, and 3CD, the viral protease, were cloned in a Saccharomyces cerevisiae inducible expression system. N-antigenic empty capsids could be isolated from the yeast cell extract provided that pirodavir, a capsid-binding compound and capsid stabilizer, was added during the induction period and during purification. Purification was by immunoaffinity chromatography. The purified empty capsids had the same immunogenicity as poliovirus virions. The techniques described might be useful for the production of new non-infectious vaccines.


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