J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


J Gen Virol 78 (1997), 1829-1832
© 1997 Society for General Microbiology

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rombaut, B.
Right arrow Articles by Jore, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rombaut, B.
Right arrow Articles by Jore, J. P.
Agricola
Right arrow Articles by Rombaut, B.
Right arrow Articles by Jore, J. P.

Journal of General Virology, Vol 78, 1829-1832, Copyright © 1997 by Society for General Microbiology


ARTICLES

Immunogenic, non-infectious polio subviral particles synthesized in Saccharomyces cerevisiae

B Rombaut and JP Jore
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Brussels, Belgium. brombaut@mich.vub.ac.be

Polioviral genes coding for P1, the precursor for the structural proteins, and 3CD, the viral protease, were cloned in a Saccharomyces cerevisiae inducible expression system. N-antigenic empty capsids could be isolated from the yeast cell extract provided that pirodavir, a capsid-binding compound and capsid stabilizer, was added during the induction period and during purification. Purification was by immunoaffinity chromatography. The purified empty capsids had the same immunogenicity as poliovirus virions. The techniques described might be useful for the production of new non-infectious vaccines.


This article has been cited by other articles:


Home page
J. Virol.Home page
S. Goodwin, T. J. Tuthill, A. Arias, R. A. Killington, and D. J. Rowlands
Foot-and-Mouth Disease Virus Assembly: Processing of Recombinant Capsid Precursor by Exogenous Protease Induces Self-Assembly of Pentamers In Vitro in a Myristoylation-Dependent Manner
J. Virol., November 1, 2009; 83(21): 11275 - 11282.
[Abstract] [Full Text] [PDF]


Home page
CVIHome page
Y.-J. Ko, K.-S. Choi, J.-J. Nah, D. J. Paton, J.-K. Oem, G. Wilsden, S.-Y. Kang, N.-I. Jo, J.-H. Lee, J.-H. Kim, et al.
Noninfectious Virus-Like Particle Antigen for Detection of Swine Vesicular Disease Virus Antibodies in Pigs by Enzyme-Linked Immunosorbent Assay
Clin. Vaccine Immunol., August 1, 2005; 12(8): 922 - 929.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1997 by the Society for General Microbiology.