Journal of General Virology, Vol 78, 1971-1975, Copyright © 1997 by Society for General Microbiology

ARTICLES

Apoptosis of cord blood T lymphocytes by herpes simplex virus type 1

M Ito, W Koide, M Watanabe, H Kamiya and M Sakurai
Department of Pediatrics, Mie University School of Medicine, Tsu, Japan. virus@clin.medic.mie-u.ac.jp

We investigated apoptosis induced by herpes simplex virus type 1 (HSV- 1) in cord blood T lymphocytes by using agarose gel electrophoresis, DNA content analysis and the terminal deoxytransferase (TdT)-mediated dUTP nick end-labelling (TUNEL) method. DNA fragmentation and the hypodiploid fraction in the cell cycle were both increased in HSV-1- infected CD4 and CD8 lymphocytes stimulated with phytohaemagglutinin (PHA) compared to mock-infected lymphocytes. The percentage of cells in the S phase was decreased in HSV-1-infected CD4 and CD8 lymphocytes. HSV-1 antigen, glycoprotein D (gD) and regulatory protein ICP27 were detected in 8-18% of the hypodiploid fraction of PHA-stimulated, HSV-1- infected lymphocytes. Apoptosis was induced not only in HSV-1 antigen- expressing cells but also in cells not expressing detectable viral proteins. Addition of anti-Fas antibody, anti-Fas-ligand antibody or a mixture of both had no effect on HSV-1-induced apoptosis, indicating that the Fas-Fas-ligand pathway did not contribute to HSV-1-induced apoptosis.

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