Journal of General Virology, Vol 78, 2077-2083, Copyright © 1997 by Society for General Microbiology

ARTICLES

Complementation of a potato virus X mutant mediated by bombardment of plant tissues with cloned viral movement protein genes

SYu Morozov, ON Fedorkin, G Juttner, J Schiemann, DC Baulcombe and JG Atabekov
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.

Microprojectile bombardment was used to examine the transport function of the 25 kDa movement protein (MP) encoded in the triple gene block of potato virus X (PVX). A 25 kDa MP-defective full-length cloned PVX genome carrying a beta-glucuronidase (GUS) reporter gene was co- bombarded with 35S promoter constructs containing either the 25 kDa MP gene of wild-type PVX, the MP gene of either of two tobamoviruses (tomato mosaic virus or crucifer tobamovirus), red clover necrotic mosaic dianthovirus (RCNMV) or brome mosaic bromovirus (BMV). When inoculated alone, the MP-defective PVX was unable to move out of the inoculated cell, as visualized by in situ staining for GUS activity. However, cell-to-cell movement of the mutant PVX genome was restored by co-inoculation with 35S constructs containing the MP cDNA of PVX, either tobamovirus or RCNMV. The BMV MP construct did not complement movement of the defective PVX. These results show that co-bombardment of cDNA of an MP-defective virus with plasmids designed to express MP of other viruses could be used as a fast and simple method for transcomplementation experiments.

This article has been cited by other articles:

				M. V. Schepetilnikov, A. G. Solovyev, E. N. Gorshkova, J. Schiemann, A. I. Prokhnevsky, V. V. Dolja, and S. Y. Morozov Intracellular Targeting of a Hordeiviral Membrane-Spanning Movement Protein: Sequence Requirements and Involvement of an Unconventional Mechanism J. Virol., February 1, 2008; 82(3): 1284 - 1293. [Abstract] [Full Text] [PDF]

				M.-K. Lin, C.-C. Hu, N.-S. Lin, B.-Y. Chang, and Y.-H. Hsu Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1357 - 1367. [Abstract] [Full Text] [PDF]

				M. V. Schepetilnikov, U. Manske, A. G. Solovyev, A. A. Zamyatnin Jr, J. Schiemann, and S. Yu. Morozov The hydrophobic segment of Potato virus X TGBp3 is a major determinant of the protein intracellular trafficking J. Gen. Virol., August 1, 2005; 86(8): 2379 - 2391. [Abstract] [Full Text] [PDF]

				P. Ajjikuttira, C.-S. Loh, and S.-M. Wong Reciprocal function of movement proteins and complementation of long-distance movement of Cymbidium mosaic virus RNA by Odontoglossum ringspot virus coat protein J. Gen. Virol., May 1, 2005; 86(5): 1543 - 1553. [Abstract] [Full Text] [PDF]

				Y.-W. Huang, Y.-F. Geng, X.-B. Ying, X.-Y. Chen, and R.-X. Fang Identification of a Movement Protein of Rice Yellow Stunt Rhabdovirus J. Virol., February 15, 2005; 79(4): 2108 - 2114. [Abstract] [Full Text] [PDF]

				N. E. Yelina, T. N. Erokhina, N. I. Lukhovitskaya, E. A. Minina, M. V. Schepetilnikov, D.-E. Lesemann, J. Schiemann, A. G. Solovyev, and S. Yu. Morozov Localization of Poa semilatent virus cysteine-rich protein in peroxisomes is dispensable for its ability to suppress RNA silencing J. Gen. Virol., February 1, 2005; 86(2): 479 - 489. [Abstract] [Full Text] [PDF]

				Y. Li, Y. M. Bao, C. H. Wei, Z. S. Kang, Y. W. Zhong, P. Mao, G. Wu, Z. L. Chen, J. Schiemann, and R. S. Nelson Rice Dwarf Phytoreovirus Segment S6-Encoded Nonstructural Protein Has a Cell-to-Cell Movement Function J. Virol., May 15, 2004; 78(10): 5382 - 5389. [Abstract] [Full Text] [PDF]

				M.-K. Lin, B.-Y. Chang, J.-T. Liao, N.-S. Lin, and Y.-H. Hsu Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of Bamboo mosaic virus are essential for virus movement J. Gen. Virol., January 1, 2004; 85(1): 251 - 259. [Abstract] [Full Text] [PDF]

				H. An, U. Melcher, P. Doss, M. Payton, A. C. Guenzi, and J. Verchot-Lubicz Evidence that the 37 kDa protein of Soil-borne wheat mosaic virus is a virus movement protein J. Gen. Virol., November 1, 2003; 84(11): 3153 - 3163. [Abstract] [Full Text] [PDF]

				S. Yu. Morozov and A. G. Solovyev Triple gene block: modular design of a multifunctional machine for plant virus movement J. Gen. Virol., June 1, 2003; 84(6): 1351 - 1366. [Abstract] [Full Text] [PDF]

				E. N. Gorshkova, T. N. Erokhina, T. A. Stroganova, N. E. Yelina, A. A. Zamyatnin Jr, N. O. Kalinina, J. Schiemann, A. G. Solovyev, and S. Yu. Morozov Immunodetection and fluorescent microscopy of transgenically expressed hordeivirus TGBp3 movement protein reveals its association with endoplasmic reticulum elements in close proximity to plasmodesmata J. Gen. Virol., April 1, 2003; 84(4): 985 - 994. [Abstract] [Full Text] [PDF]

				A. A. Zamyatnin Jr,, A. G. Solovyev, A. A. Sablina, A. A. Agranovsky, L. Katul, H. J. Vetten, J. Schiemann, A. E. Hinkkanen, K. Lehto, and S. Yu. Morozov Dual-colour imaging of membrane protein targeting directed by poa semilatent virus movement protein TGBp3 in plant and mammalian cells J. Gen. Virol., March 1, 2002; 83(3): 651 - 662. [Abstract] [Full Text] [PDF]

				D. M. Lawrence and A. O. Jackson Interactions of the TGB1 Protein during Cell-to-Cell Movement of Barley Stripe Mosaic Virus J. Virol., September 15, 2001; 75(18): 8712 - 8723. [Abstract] [Full Text] [PDF]

				R. Dasgupta, B. H. Garcia II, and R. M. Goodman Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes PNAS, April 5, 2001; (2001) 81288198. [Abstract] [Full Text]

				O. N. Fedorkin, A. G. Solovyev, N. E. Yelina, A. A. Zamyatnin Jr, R. A. Zinovkin, K. Mäkinen, J. Schiemann, and S. Yu. Morozov Cell-to-cell movement of potato virus X involves distinct functions of the coat protein J. Gen. Virol., February 1, 2001; 82(2): 449 - 458. [Abstract] [Full Text]

				A. Merits, O. N. Fedorkin, D. Guo, N. O. Kalinina, and S. Yu. Morozov Activities associated with the putative replication initiation protein of Coconut foliar decay virus, a tentative member of the genus Nanovirus J. Gen. Virol., December 1, 2000; 81(12): 3099 - 3106. [Abstract] [Full Text]

				M. Varrelmann, L. Palkovics, and E. Maiss Transgenic or Plant Expression Vector-Mediated Recombination of Plum Pox Virus J. Virol., August 15, 2000; 74(16): 7462 - 7469. [Abstract] [Full Text]

				M. Varrelmann and E. Maiss Mutations in the coat protein gene of Plum pox virus suppress particle assembly, heterologous encapsidation and complementation in transgenic plants of Nicotiana benthamiana J. Gen. Virol., March 1, 2000; 81(3): 567 - 576. [Abstract] [Full Text]

				S. G. Lazarowitz and R. N. Beachy Viral Movement Proteins as Probes for Intracellular and Intercellular Trafficking in Plants PLANT CELL, April 1, 1999; 11(4): 535 - 548. [Full Text]

				J. Verchot, S. M. Angell, and D. C. Baulcombe In Vivo Translation of the Triple Gene Block of Potato Virus X Requires Two Subgenomic mRNAs J. Virol., October 1, 1998; 72(10): 8316 - 8320. [Abstract] [Full Text] [PDF]

				R. Dasgupta, B. H. Garcia II, and R. M. Goodman Systemic spread of an RNA insect virus in plants expressing plant viral movement protein genes PNAS, April 24, 2001; 98(9): 4910 - 4915. [Abstract] [Full Text] [PDF]