Journal of General Virology, Vol 78, 2089-2093, Copyright © 1997 by Society for General Microbiology

ARTICLES

Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus

DT Kasteel, NN van der Wel, KA Jansen, RW Goldbach and JW van Lent
Department of Virology, Agricultural University Wageningen, The Netherlands.

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

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