Journal of General Virology, Vol 78, 2171-2178, Copyright © 1997 by Society for General Microbiology

ARTICLES

Identification of a variant B-specific neutralizing epitope on glycoprotein H of human herpesvirus-6

K Takeda, M Haque, T Sunagawa, T Okuno, Y Isegawa and K Yamanishi
Department of Microbiology, Osaka University Medical School, Osaka University, Japan.

We have identified the human herpesvirus-6 variant B (HHV-6B)-specific neutralizing epitope on glycoprotein H (gH) which is recognized by monoclonal antibody (MAb) OHV3, with complement-independent neutralizing activity. HHV-6 gHs from HHV-6A (strain U1102) and HHV-6B (strain HST) were expressed in a T7-vaccinia virus transient expression system. OHV3 reacted with HST gH, but not with U1102 gH, in an immunoprecipitation assay and an indirect immunofluorescence assay. In addition, OHV3 reacted with chimeric gHs, formed between U1102 gH and HST gH, containing amino acids 272 to 422 of HST gH. Sequence comparison between U1102 and HST showed seven amino acid differences in this region. Site-specific mutations were introduced into these positions and then reactivity against OHV3 was investigated. The arginine at position 389 of HST gH was shown to be a determinant of the HHV-6B-specific reactivity of OHV3.

This article has been cited by other articles:

				M. Takemoto, Y. Mori, K. Ueda, K. Kondo, and K. Yamanishi Productive human herpesvirus 6 infection causes aberrant accumulation of p53 and prevents apoptosis J. Gen. Virol., April 1, 2004; 85(4): 869 - 879. [Abstract] [Full Text] [PDF]

				F. Santoro, H. L. Greenstone, A. Insinga, M. K. Liszewski, J. P. Atkinson, P. Lusso, and E. A. Berger Interaction of Glycoprotein H of Human Herpesvirus 6 with the Cellular Receptor CD46 J. Biol. Chem., July 3, 2003; 278(28): 25964 - 25969. [Abstract] [Full Text] [PDF]

				M. Haque, D. A. Davis, V. Wang, I. Widmer, and R. Yarchoan Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Contains Hypoxia Response Elements: Relevance to Lytic Induction by Hypoxia J. Virol., June 15, 2003; 77(12): 6761 - 6768. [Abstract] [Full Text] [PDF]

				Y. Mori, X. Yang, P. Akkapaiboon, T. Okuno, and K. Yamanishi Human Herpesvirus 6 Variant A Glycoprotein H-Glycoprotein L-Glycoprotein Q Complex Associates with Human CD46 J. Virol., April 15, 2003; 77(8): 4992 - 4999. [Abstract] [Full Text] [PDF]

				Y. Mori, P. Akkapaiboon, X. Yang, and K. Yamanishi The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope J. Virol., February 15, 2003; 77(4): 2452 - 2458. [Abstract] [Full Text] [PDF]

				C. Ochsenbauer-Jambor, S. E. Delos, M. A. Accavitti, J. M. White, and E. Hunter Novel Monoclonal Antibody Directed at the Receptor Binding Site on the Avian Sarcoma and Leukosis Virus Env Complex J. Virol., June 27, 2002; 76(15): 7518 - 7527. [Abstract] [Full Text] [PDF]

				Y. Mori, T. Seya, H. L. Huang, P. Akkapaiboon, P. Dhepakson, and K. Yamanishi Human Herpesvirus 6 Variant A but Not Variant B Induces Fusion from Without in a Variety of Human Cells through a Human Herpesvirus 6 Entry Receptor, CD46 J. Virol., June 5, 2002; 76(13): 6750 - 6761. [Abstract] [Full Text] [PDF]

				Y. Mori, P. Dhepakson, T. Shimamoto, K. Ueda, Y. Gomi, H. Tani, Y. Matsuura, and K. Yamanishi Expression of Human Herpesvirus 6B rep within Infected Cells and Binding of Its Gene Product to the TATA-Binding Protein In Vitro and In Vivo J. Virol., July 1, 2000; 74(13): 6096 - 6104. [Abstract] [Full Text]

				Y. Isegawa, T. Mukai, K. Nakano, M. Kagawa, J. Chen, Y. Mori, T. Sunagawa, K. Kawanishi, J. Sashihara, A. Hata, et al. Comparison of the Complete DNA Sequences of Human Herpesvirus 6 Variants A and B J. Virol., October 1, 1999; 73(10): 8053 - 8063. [Abstract] [Full Text] [PDF]

				R. Anderson and U. Gompels N- and C-terminal external domains of human herpesvirus-6 glycoprotein H affect a fusion-associated conformation mediated by glycoprotein L binding the N terminus J. Gen. Virol., June 1, 1999; 80(6): 1485 - 1494. [Abstract]