J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Inglis, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Inglis, S.
Agricola
Right arrow Articles by Zhang, X.
Right arrow Articles by Inglis, S.

Journal of General Virology, Vol 79, 125-131, Copyright © 1998 by Society for General Microbiology

ARTICLES

An efficient selection system for packaging herpes simplex virus amplicons

X Zhang, H O'Shea, C Entwisle, M Boursnell, S Efstathiou and S Inglis
Cantab Pharmaceuticals Research Ltd, Cambridge, UK. xzhang@cantab.co.uk

Due to their simplicity and flexibility of genomic construction, herpes simplex virus (HSV) amplicon-based vectors are attractive vehicles for gene delivery. However, a significant problem faced in the generation of amplicon stocks is the low amplicon to helper virus (A/H) ratio. In order to improve the proportion of amplicons generated, a selection system for amplicon production was developed in which the HSV thymidine kinase (TK) gene is inserted into an amplicon plasmid and an HSV mutant with both TK and glycoprotein H (gH) genes deleted is used as a helper virus. Using a protocol in which amplicon stocks are passaged 2-3 times in BHK cells of TK- and gH+ genotype in the presence of selection medium containing methotrexate, stock preparations with high A/H ratio (up to 5:1) and high amplicon titre (>1 x 10(9) infectious units/ml) were generated. In vitro characterization demonstrated that a high level of biologically functional products can be efficiently produced from these amplicon constructs.


This article has been cited by other articles:


Home page
 J. Virol.Home page
X. Fu, H. Wang, and X. Zhang
High-Frequency Intermolecular Homologous Recombination during Herpes Simplex Virus-Mediated Plasmid DNA Replication
J. Virol., May 13, 2002; 76(12): 5866 - 5874.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Fu and X. Zhang
Potent Systemic Antitumor Activity from an Oncolytic Herpes Simplex Virus of Syncytial Phenotype
Cancer Res., April 1, 2002; 62(8): 2306 - 2312.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Wang, X. Fu, and X. Zhang
Isomerization of a Uniquely Designed Amplicon during Herpes Simplex Virus-Mediated Replication
J. Virol., November 1, 2001; 75(21): 10505 - 10510.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. A. Stavropoulos and C. A. Strathdee
An Enhanced Packaging System for Helper-Dependent Herpes Simplex Virus Vectors
J. Virol., September 1, 1998; 72(9): 7137 - 7143.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1998 by the Society for General Microbiology.