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Journal of General Virology, Vol 79, 133-141, Copyright © 1998 by Society for General Microbiology

ARTICLES

Characterization of the bovine herpesvirus 1 UL8 gene and gene products

J Schmitt and GM Keil
Institute of Molecular and Cellular Virology, Friedrich-Loeffler- Institutes, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany.

The bovine herpesvirus 1 (BHV-1) strain Schonboken UL8 gene and the 5' flanking region were sequenced. Comparison of the UL8 ORF with the previously reported UL8 ORF of BHV-1 strain Cooper revealed significant differences that were mainly due to three frame-shifted segments. Reanalysis of the Cooper sequence after isolation of the respective segments from genomic DNA by PCR did not confirm the discrepancies; on the contrary, our results indicate a high degree of sequence conservation between the UL8 proteins of different BHV-1 isolates. A monospecific antiserum, raised against a bacterially expressed TrpE-UL8 fusion protein, identified the 80 kDa apparent molecular mass UL8 polypeptide which is localized in the nucleus of infected cells. Analysis of transcripts and time-course studies demonstrated that the UL8 protein is translated from a delayed-early expressed 3.1 kb polyadenylated mRNA which initiates within the UL9 ORF.




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Copyright © 1998 by the Society for General Microbiology.