J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Astori, M.
Right arrow Articles by Karapetian, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Astori, M.
Right arrow Articles by Karapetian, O.
Agricola
Right arrow Articles by Astori, M.
Right arrow Articles by Karapetian, O.

Journal of General Virology, Vol 79, 57-60, Copyright © 1998 by Society for General Microbiology

ARTICLES

Antibodies to the C-terminal dipeptide of mouse mammary tumor virus [MMTV(SW)] superantigen effectively inhibit T-cell activation in vivo

M Astori and O Karapetian
Swiss Institute for Experimental Cancer Research and Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland. mastori@eliot.unil.ch

BALB/c mice were immunized against the 19 C-terminal amino acids of the mouse mammary tumour virus [MMTV(SW)] superantigen, either actively with the recombinant ORF19 protein or passively by subcutaneous implantation of 6E1.8 hybridoma cells. Protection against the MMTV(SW) superantigen-induced activation of specific T cells was obtained in both immunizations. Whereas the monoclonal antibody provided complete protection, similar antibody titres showed less effective protection in actively immunized mice. This was due to the presence of a histidine tag on the recombinant ORF19 protein preventing immune recognition of the C-terminal amino acids during immunization. Preincubation of the 6E1.8 monoclonal antibodies with modified ORF19 peptides at their C terminus established specific recognition of the last two amino acids of MMTV(SW) superantigen by this antibody, and showed that these amino acids are critical in the interaction between the MMTV(SW) superantigen and the Vbeta element of the T-cell receptor.


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
P. R. Etkind, A. F. R. Stewart, T. Dorai, D. J. Purcell, and P. H. Wiernik
Clonal Isolation of Different Strains of Mouse Mammary Tumor Virus-Like DNA Sequences from Both the Breast Tumors and Non-Hodgkin's Lymphomas of Individual Patients Diagnosed with Both Malignancies
Clin. Cancer Res., September 1, 2004; 10(17): 5656 - 5664.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Mpandi, L. A. Otten, C. Lavanchy, H. Acha-Orbea, and D. Finke
Passive Immunization with Neutralizing Antibodies Interrupts the Mouse Mammary Tumor Virus Life Cycle
J. Virol., September 1, 2003; 77(17): 9369 - 9377.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1998 by the Society for General Microbiology.