Journal of General Virology, Vol 79, 2481-2489, Copyright © 1998 by Society for General Microbiology

ARTICLES

Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus

H Attoui, RN Charrel, F Billoir, JF Cantaloube, P de Micco and X de Lamballerie
Laboratoire de Virologie Moleculaire, Tropicale et Transfusionnelle, Faculte de Medecine de Marseille, Universite de la Mediterranee, Marseille, France.

In this study, the basis for the classification of virus isolates grouped within the genus Coltivirus, family Reoviridae, is discussed. Sequences of dsRNA segments from American (segments 9-12), European (segment 12) and Asian (segments 7-12) isolates were characterized and polythetic criteria were defined for their taxonomic classification. These criteria (including sequence analysis) permitted the different species to be distinguished and classified into two groups. In both groups, subgroups were defined according to the degree of homology between the genomic sequences. American and European isolates are classified within group A, which includes subgroups A1 (Colorado tick fever virus species) and A2 (Eyach virus species). Asian isolates are classified in group B, which includes subgroups B1 (JKT-7075 virus species) and B2 (JKT-6423 virus species). The proteins encoded by the sequenced genomic segments were analysed. This allowed the identification of dsRNA binding domains in the proteins encoded by segment 8 of subgroup B1 isolates and segment 12 of subgroup B2 isolates. A conserved pattern of amino acids in segment 7 of group B isolates matched sequences found in the catalytic domains of protein kinases.

This article has been cited by other articles:

				H. Attoui, F. M. Jaafar, M. Belhouchet, P. de Micco, X. de Lamballerie, and C. P. D. Brussaard Micromonas pusilla reovirus: a new member of the family Reoviridae assigned to a novel proposed genus (Mimoreovirus). J. Gen. Virol., May 1, 2006; 87(Pt 5): 1375 - 1383. [Abstract] [Full Text] [PDF]

				B. T. Poulos, K. F. J. Tang, C. R. Pantoja, J. R. Bonami, and D. V. Lightner Purification and characterization of infectious myonecrosis virus of penaeid shrimp. J. Gen. Virol., April 1, 2006; 87(Pt 4): 987 - 996. [Abstract] [Full Text] [PDF]

				H. Attoui, F. M. Jaafar, M. Belhouchet, S. Tao, B. Chen, G. Liang, R. B. Tesh, P. de Micco, and X. de Lamballerie Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells J. Gen. Virol., January 1, 2006; 87(1): 199 - 208. [Abstract] [Full Text] [PDF]

				F. M. Jaafar, H. Attoui, P. P. C. Mertens, P. de Micco, and X. de Lamballerie Structural organization of an encephalitic human isolate of Banna virus (genus Seadornavirus, family Reoviridae) J. Gen. Virol., April 1, 2005; 86(4): 1147 - 1157. [Abstract] [Full Text] [PDF]

				F. M. Jaafar, H. Attoui, P. de Micco, and X. de Lamballerie Termination and read-through proteins encoded by genome segment 9 of Colorado tick fever virus J. Gen. Virol., August 1, 2004; 85(8): 2237 - 2244. [Abstract] [Full Text] [PDF]

				L. R. SAUNDERS and G. N. BARBER The dsRNA binding protein family: critical roles, diverse cellular functions FASEB J, June 1, 2003; 17(9): 961 - 983. [Abstract] [Full Text] [PDF]

				A. C. Potgieter, A. D. Steele, and A. A. van Dijk Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes J. Gen. Virol., September 1, 2002; 83(9): 2215 - 2223. [Abstract] [Full Text] [PDF]

				H. Attoui, F. Billoir, P. Biagini, P. de Micco, and X. de Lamballerie Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae J. Gen. Virol., June 1, 2000; 81(6): 1507 - 1515. [Abstract] [Full Text]

				R. L. Harrison and B. C. Bonning The nucleopolyhedroviruses of Rachiplusia ou and Anagrapha falcifera are isolates of the same virus J. Gen. Virol., October 1, 1999; 80(10): 2793 - 2798. [Abstract] [Full Text]