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Journal of General Virology, Vol 79, 2753-2760, Copyright © 1998 by Society for General Microbiology

ARTICLES

PEF-1, an epithelial cell transcription factor which activates the long control region of human papillomavirus type 16, is glycosylated with N- acetylglucosamine

D Fergusson and MS Campo
The Beatson Institute for Cancer Research, Glasgow, UK. D.Fergusson@udcf.gla.ac.uk

Infection by human papillomavirus type 16 (HPV-16) has been linked to cervical cancer. The transcription of viral genes in HPV-16 is partially controlled by a number of cellular transcription factors. We have previously identified a novel cellular transcription factor, PEF- 1, from its ability to interact with the long control region (LCR) of HPV-16. This factor has a molecular mass of about 110 kDa and binds to a GC-rich sequence in the section of the LCR responsible for cell type- specific transcription from viral DNA. The factor Sp1 has similar properties and also interacts with the HPV-16 LCR. We show that PEF-1 and Sp1 are distinct transcription factors: they recognize different DNA sequences, have different electrophoretic mobilities and different glycosylation patterns. Sp1 is O-glycosylated while PEF-1 appears to have a novel type of glycosylation, as shown by the interaction with pokeweed lectin and by the inhibition of this interaction by tunicamycin.


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J. H. Lee, S. M. P. Yi, M. E. Anderson, K. L. Berger, M. J. Welsh, A. J. Klingelhutz, and M. A. Ozbun
Propagation of infectious human papillomavirus type 16 by using an adenovirus and Cre/LoxP mechanism
PNAS, February 17, 2004; 101(7): 2094 - 2099.
[Abstract] [Full Text] [PDF]


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