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Journal of General Virology, Vol 79, 333-337, Copyright © 1998 by Society for General Microbiology

ARTICLES

Intracellular production of African horsesickness virus core-like particles by expression of the two major core proteins, VP3 and VP7, in insect cells

S Maree, S Durbach and H Huismans
Biochemistry Division, Onderstepoort Veterinary Institute, South Africa.

To gain more insight into the structure of the African horsesickness virus (AHSV) core particle, we have cloned, partially characterized and expressed the two major core proteins, VP3 and VP7, of AHSV-9. VP7 was found to be highly conserved amongst different serotypes. The VP3 and VP7 genes were subsequently expressed in insect cells by means of recombinant baculoviruses. VP7 was synthesized to very high levels and aggregated into distinctive crystals. Co-expression of VP3 and VP7 resulted in the intracellular formation of core-like particles which structurally resembled empty AHSV cores.


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A. C. Potgieter, A. D. Steele, and A. A. van Dijk
Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes
J. Gen. Virol., September 1, 2002; 83(9): 2215 - 2223.
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