J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaefer, S.
Right arrow Articles by Gerlich, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaefer, S.
Right arrow Articles by Gerlich, W. H.
Agricola
Right arrow Articles by Schaefer, S.
Right arrow Articles by Gerlich, W. H.

Journal of General Virology, Vol 79, 767-777, Copyright © 1998 by Society for General Microbiology

ARTICLES

Properties of tumour suppressor p53 in murine hepatocyte lines transformed by hepatitis B virus X protein

S Schaefer, M Seifer, T Grimmsmann, L Fink, S Wenderhold, MW Hohne and WH Gerlich
Institute of Medical Virology, Justus-Liebig University, Giessen, Germany. stephan.schaefer@viro.med.uni-giessen.de

Persistent infection by hepatitis B virus (HBV) correlates with the prevalence of hepatocellular carcinoma. It has recently been demonstrated that the complete viral genome very efficiently transforms the immortalized murine hepatocyte line FMH202 in vitro. Here it is shown that the viral transactivating protein X (HBx) is sufficient to transform FMH202 cells, albeit with lower efficiency. Clonal cell lines expressing HBx mRNA in moderate or high amounts grew in soft agar and formed tumours in nude mice. Growth efficiency in soft agar of HBx transformed cell lines was much lower than that of cell lines transformed with the complete genome, and latency of tumour induction in nude mice was significantly longer after inoculation of HBx than of HBV transformed FMH202 cell lines. A marker of complete transformation, p53, was found to be phosphorylated more strongly in HBx transfected cell lines than in controls, and a cellular kinase was found to be associated with p53 complexes from HBx transformed cell lines. p53 was of wild-type conformation and was located in the nucleus of transformed cells.


This article has been cited by other articles:


Home page
 Cancer Res.Home page
H. Tu, C. Bonura, C. Giannini, H. Mouly, P. Soussan, M. Kew, P. Paterlini-Brechot, C. Brechot, and D. Kremsdorf
Biological Impact of Natural COOH-Terminal Deletions of Hepatitis B Virus X Protein in Hepatocellular Carcinoma Tissues
Cancer Res., November 1, 2001; 61(21): 7803 - 7810.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. E. Smela, S. S. Currier, E. A. Bailey, and J. M. Essigmann
The chemistry and biology of aflatoxin B1: from mutational spectrometry to carcinogenesis
Carcinogenesis, April 1, 2001; 22(4): 535 - 545.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Diao, A. A. Khine, F. Sarangi, E. Hsu, C. Iorio, L. A. Tibbles, J. R. Woodgett, J. Penninger, and C. D. Richardson
X Protein of Hepatitis B Virus Inhibits Fas-mediated Apoptosis and Is Associated with Up-regulation of the SAPK/JNK Pathway
J. Biol. Chem., March 9, 2001; 276(11): 8328 - 8340.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Tarn, S. Lee, Y. Hu, C. Ashendel, and O. M. Andrisani
Hepatitis B Virus X Protein Differentially Activates RAS-RAF-MAPK and JNK Pathways in X-transforming Versus Non-transforming AML12 Hepatocytes
J. Biol. Chem., September 7, 2001; 276(37): 34671 - 34680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1998 by the Society for General Microbiology.