Journal of General Virology, Vol 79, 925-929, Copyright © 1998 by Society for General Microbiology

ARTICLES

Tubules containing virions are present in plant tissues infected with Commelina yellow mottle badnavirus

CP Cheng, I Tzafrir, BE Lockhart and NE Olszewski
Department of Plant Biology, Plant Molecular Genetics Institute, University of Minnesota, St Paul 55108, USA.

Tubular structures containing bacilliform virions were observed in cell- free extracts of Commelina diffusa infected with Commelina yellow mottle badnavirus (CoYMV). The exterior of the tubule reacted with antibodies to CoYMV movement protein, but not with antibodies to virus coat protein. Similar tubular structures containing bacilliform particles were also observed in ultrathin sections of CoYMV-infected C. diffusa. These tubular structures traversed the cell wall at points where this was thickened or protruded. No similar structures were observed in healthy C. diffusa. These observations support the hypothesis that the virion-containing tubular structures observed in cell-free extracts are the same as those observed in situ, that these structures are composed, at least in part, of virus movement protein, and that they play a role in the cell-to-cell trafficking of virions of CoYMV.

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