Journal of General Virology, Vol 79, 1047-1054, Copyright © 1998 by Society for General Microbiology

ARTICLES

Expression of ORF A1 of infectious bursal disease virus results in the formation of virus-like particles

A Fernandez-Arias, C Risco, S Martinez, JP Albar and JF Rodriguez
Departamento de Biologia Molecular y Celular, Centro Nacional de Biotecnologia, Cantoblanco, Madrid, Spain.

A recombinant vaccinia virus inducibly expressing ORF A1 of infectious bursal disease virus (IBDV) has been constructed and characterized. Cells infected with this recombinant virus express the IBDV polyprotein, which is proteolytically processed to give mature VP2, VP3, and VP4 polypeptides. An electron microscopy study revealed that the cytoplasm of cells infected with the recombinant virus contains abundant IBDV-like particles (VLP). These VLP form close-packed paracrystalline arrays that are specifically recognized by anti-IBDV antibodies. The size and morphology of purified VLP were found to be akin to those of authentic IBDV particles.

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