Journal of General Virology, Vol 79, 1273-1280, Copyright © 1998 by Society for General Microbiology

ARTICLES

Nucleic acid-binding properties and subcellular localization of the 3a protein of brome mosaic bromovirus

M Fujita, K Mise, Y Kajiura, K Dohi and I Furusawa
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Japan. miki@kais.kyoto-u.ac.jp

Brome mosaic bromovirus (BMV) 3a protein is required for cell-to-cell movement of the virus in host plants. The BMV 3a protein (B3a) was produced in Escherichia coli using an expression vector. Gel retardation analysis and UV cross-linking experiments demonstrated that B3a bound single-stranded RNA cooperatively without sequence specificity. Binding competition analysis showed that B3a bound to single-stranded nucleic acids more strongly than to double-stranded nucleic acids. Deletion mutagenesis located a nucleic acid-binding domain to amino acids 189-242. Western blot analysis of fractionated proteins of BMV-infected barley using monoclonal antibodies against B3a indicated that B3a may interact with membrane materials and form complexes in the cytoplasm. Immunogold labelling of thin sections of infected barley tissues revealed that B3a was associated with plasmodesmata and cytoplasmic inclusions.

This article has been cited by other articles:

				A. Takeda, M. Kaido, T. Okuno, and K. Mise The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement J. Gen. Virol., June 1, 2004; 85(6): 1751 - 1761. [Abstract] [Full Text] [PDF]

				K. M. Nurkiyanova, E. V. Ryabov, N. O. Kalinina, Y. Fan, I. Andreev, A. G. Fitzgerald, P. Palukaitis, and M. Taliansky Umbravirus-encoded movement protein induces tubule formation on the surface of protoplasts and binds RNA incompletely and non-cooperatively J. Gen. Virol., October 1, 2001; 82(10): 2579 - 2588. [Abstract] [Full Text] [PDF]

				Y. Okinaka, K. Mise, E. Suzuki, T. Okuno, and I. Furusawa The C Terminus of Brome Mosaic Virus Coat Protein Controls Viral Cell-to-Cell and Long-Distance Movement J. Virol., June 1, 2001; 75(11): 5385 - 5390. [Abstract] [Full Text]

				M. Ishikawa, M. Janda, and P. Ahlquist The 3a cell-to-cell movement gene is dispensable for cell-to-cell transmission of brome mosaic virus RNA replicons in yeast but retained over 1045-fold amplification J. Gen. Virol., September 1, 2000; 81(9): 2307 - 2311. [Abstract] [Full Text]

				Y. Matsushita, K. Hanazawa, K. Yoshioka, T. Oguchi, S. Kawakami, Y. Watanabe, M. Nishiguchi, and H. Nyunoya In vitro phosphorylation of the movement protein of tomato mosaic tobamovirus by a cellular kinase J. Gen. Virol., August 1, 2000; 81(8): 2095 - 2102. [Abstract] [Full Text]

				U. Melcher The ‘30K’ superfamily of viral movement proteins J. Gen. Virol., January 1, 2000; 81(1): 257 - 266. [Abstract] [Full Text]

				T. A. Damayanti, H. Nagano, K. Mise, I. Furusawa, and T. Okuno Brome mosaic virus defective RNAs generated during infection of barley plants J. Gen. Virol., September 1, 1999; 80(9): 2511 - 2518. [Abstract] [Full Text]

				F Osman, I Schmitz, and A. Rao Effect of C-terminal deletions in the movement protein of cowpea chlorotic mottle virus on cell-to-cell and long-distance movement J. Gen. Virol., June 1, 1999; 80(6): 1357 - 1365. [Abstract]

				C. Wung, Y. Hsu, D. Liou, W. Huang, N. Lin, and B. Chang Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic potexvirus J. Gen. Virol., May 1, 1999; 80(5): 1119 - 1126. [Abstract]