Journal of General Virology, Vol 79, 989-999, Copyright © 1998 by Society for General Microbiology

ARTICLES

Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus

B Pirzadeh and S Dea
Centre de recherche en virologie, Institut Armand-Frappier, Universite du Quebec, Laval-des-Rapides, Canada.

The ORF5-encoded major envelope glycoprotein (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) is one of the three major structural proteins of this virus. While some porcine convalescent sera and monoclonal antibodies directed against GP4 and GP5 have the capacity to neutralize the virus in vitro, the protein specificity of porcine neutralizing sera has not yet been established. DNA immunization with a plasmid encoding GP5 of PRRSV, under the control of a human cytomegalovirus promoter, induced anti-GP5-specific neutralizing antibodies in pigs and BALB/c mice. The GP5 protein specificity of neutralizing sera was confirmed by immunoblotting and ELISA. Peripheral blood mononuclear cells obtained from DNA-vaccinated pigs underwent blastogenic transformation in the presence of E. coli- expressed recombinant ORF5-encoded protein, indicating the specificity of the cellular immune response to GP5. Following a massive intratracheal challenge with the virulent IAF-Klop strain of PRRSV, DNA- vaccinated pigs were protected from generalized viraemia and the development of typical macroscopic lung lesions that were observed in unvaccinated, virus-challenged controls, as well as in pigs that were immunized with E. coli-expressed GST-ORF5 recombinant fusion protein. Interstitial pneumonitis and broncho-alveolitis were remarkably milder in DNA-vaccinated animals. These results suggest that the GP5 of PRRSV is a good candidate for a subunit recombinant-type vaccine.

This article has been cited by other articles:

				O. J. Lopez, M. F. Oliveira, E. A. Garcia, B. J. Kwon, A. Doster, and F. A. Osorio Protection against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection through Passive Transfer of PRRSV-Neutralizing Antibodies Is Dose Dependent Clin. Vaccine Immunol., March 1, 2007; 14(3): 269 - 275. [Abstract] [Full Text] [PDF]

				E. H. J. Wissink, H. A. R. van Wijk, M. V. Kroese, E. Weiland, J. J. M. Meulenberg, P. J. M. Rottier, and P. A. van Rijn The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain J. Gen. Virol., June 1, 2003; 84(6): 1535 - 1543. [Abstract] [Full Text] [PDF]

				E. Mateu, M. Martin, and D. Vidal Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type porcine reproductive and respiratory virus strains in Spain J. Gen. Virol., March 1, 2003; 84(3): 529 - 534. [Abstract] [Full Text] [PDF]

				M. B. Oleksiewicz, A. Botner, and P. Normann Porcine B-cells recognize epitopes that are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus J. Gen. Virol., June 1, 2002; 83(6): 1407 - 1418. [Abstract] [Full Text] [PDF]

				C.-C. Chang, K.-J. Yoon, J. J. Zimmerman, K. M. Harmon, P. M. Dixon, C. M. T. Dvorak, and M. P. Murtaugh Evolution of Porcine Reproductive and Respiratory Syndrome Virus during Sequential Passages in Pigs J. Virol., April 16, 2002; 76(10): 4750 - 4763. [Abstract] [Full Text] [PDF]

				M. Ostrowski, J. A. Galeota, A. M. Jar, K. B. Platt, F. A. Osorio, and O. J. Lopez Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 Ectodomain J. Virol., April 3, 2002; 76(9): 4241 - 4250. [Abstract] [Full Text] [PDF]

				S. Alonso, I. Sola, J. P. Teifke, I. Reimann, A. Izeta, M. Balasch, J. Plana-Duran, R. J. M. Moormann, and L. Enjuanes In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes J. Gen. Virol., March 1, 2002; 83(3): 567 - 579. [Abstract] [Full Text] [PDF]

				M. J. Rodriguez, J. Sarraseca, J. Fominaya, E. Cortés, A. Sanz, and J. I. Casal Identification of an immunodominant epitope in the C terminus of glycoprotein 5 of porcine reproductive and respiratory syndrome virus J. Gen. Virol., May 1, 2001; 82(5): 995 - 999. [Abstract] [Full Text]

				S. Indik, L. Valícek, D. Klein, and J. Klánová Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus J. Gen. Virol., October 1, 2000; 81(10): 2497 - 2502. [Abstract] [Full Text]

				S. Harpin, D. J. Hurley, M. Mbikay, B. Talbot, and Y. Elazhary Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2 J. Gen. Virol., December 1, 1999; 80(12): 3137 - 3144. [Abstract] [Full Text]