J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grassauer, A.
Right arrow Articles by Muster, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grassauer, A.
Right arrow Articles by Muster, T.
Agricola
Right arrow Articles by Grassauer, A.
Right arrow Articles by Muster, T.

Journal of General Virology, Vol 79, 1405-1409, Copyright © 1998 by Society for General Microbiology

ARTICLES

A host restriction-based selection system for influenza haemagglutinin transfectant viruses

A Grassauer, AY Egorov, B Ferko, I Romanova, H Katinger and T Muster
Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria.

During the 1996 influenza epidemic in Vienna we obtained influenza A virus specimens (Vienna/47/96, Vienna/81/96) which grow efficiently in African green monkey kidney (Vero) cells but not in embryonated chicken eggs. Amplification of the specimens in Vero cells resulted in progeny that agglutinated human but not chicken erythrocytes. Reassortment analysis suggested that the haemagglutinin (HA) might be responsible for the host restriction. Vero cells were infected with the Vienna/47/96 virus and then transfected with reconstituted ribonucleoprotein complexes containing HA genes from egg-adapted strains. Subsequent selective passages in embryonated chicken eggs resulted in selection of transfectant viruses, growing in eggs and containing the transfected HAs. The results demonstrate that host restriction of the Vero-adapted Vienna/47/96 virus is due to its HA. Moreover, the experiments showed that the Vienna/47/96 strain can be used as helper virus for reverse genetics experiments.


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
D. Y. Oh, I. G. Barr, J. A. Mosse, and K. L. Laurie
MDCK-SIAT1 Cells Show Improved Isolation Rates for Recent Human Influenza Viruses Compared to Conventional MDCK Cells
J. Clin. Microbiol., July 1, 2008; 46(7): 2189 - 2194.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. Lu, H. Zhou, D. Ye, G. Kemble, and H. Jin
Improvement of Influenza A/Fujian/411/02 (H3N2) Virus Growth in Embryonated Chicken Eggs by Balancing the Hemagglutinin and Neuraminidase Activities, Using Reverse Genetics
J. Virol., June 1, 2005; 79(11): 6763 - 6771.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Ozaki, E. A. Govorkova, C. Li, X. Xiong, R. G. Webster, and R. J. Webby
Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics
J. Virol., February 15, 2004; 78(4): 1851 - 1857.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
K. V. Rowley, R. Harvey, and W. S. Barclay
Isolation and characterization of a transfectant influenza B virus altered in RNA segment 6
J. Gen. Virol., September 1, 1999; 80(9): 2353 - 2359.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1998 by the Society for General Microbiology.