Journal of General Virology, Vol 79, 1671-1675, Copyright © 1998 by Society for General Microbiology

ARTICLES

Adenovirus core protein V is delivered by the invading virus to the nucleus of the infected cell and later in infection is associated with nucleoli

DA Matthews and WC Russell
School of Biomedical Sciences, University of St Andrews, UK.

A 'west-Western' blotting procedure indicates that adenovirus core protein V is linked to the capsid via protein VI. Double-labelling techniques employing confocal microscopy and immunofluorescence suggest that this linkage is disrupted following infection and penetration of the host cell. Protein V enters the nucleus presumably still in association with the other core proteins attached to the virus genome. Later in infection protein V rapidly accumulates in the nucleus in close association with nucleoli.

This article has been cited by other articles:

				A. E. Tollefson, B. Ying, K. Doronin, P. D. Sidor, and W. S. M. Wold Identification of a New Human Adenovirus Protein Encoded by a Novel Late l-Strand Transcription Unit J. Virol., December 1, 2007; 81(23): 12918 - 12926. [Abstract] [Full Text] [PDF]

				J. R. Boyne and A. Whitehouse Nucleolar trafficking is essential for nuclear export of intronless herpesvirus mRNA PNAS, October 10, 2006; 103(41): 15190 - 15195. [Abstract] [Full Text] [PDF]

				H. Wodrich, A. Cassany, M. A. D'Angelo, T. Guan, G. Nemerow, and L. Gerace Adenovirus Core Protein pVII Is Translocated into the Nucleus by Multiple Import Receptor Pathways J. Virol., October 1, 2006; 80(19): 9608 - 9618. [Abstract] [Full Text] [PDF]

				F. J. Lawrence, B. McStay, and D. A. Matthews Nucleolar protein upstream binding factor is sequestered into adenovirus DNA replication centres during infection without affecting RNA polymerase I location or ablating rRNA synthesis J. Cell Sci., June 15, 2006; 119(12): 2621 - 2631. [Abstract] [Full Text] [PDF]

				J. C. Grieger, S. Snowdy, and R. J. Samulski Separate Basic Region Motifs within the Adeno-Associated Virus Capsid Proteins Are Essential for Infectivity and Assembly. J. Virol., June 1, 2006; 80(11): 5199 - 5210. [Abstract] [Full Text] [PDF]

				J. S. Johnson, Y. N. Osheim, Y. Xue, M. R. Emanuel, P. W. Lewis, A. Bankovich, A. L. Beyer, and D. A. Engel Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A J. Virol., June 15, 2004; 78(12): 6459 - 6468. [Abstract] [Full Text] [PDF]

				T. W. R. Lee, F. J. Lawrence, V. Dauksaite, G. Akusjarvi, G. E. Blair, and D. A. Matthews Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins J. Gen. Virol., January 1, 2004; 85(1): 185 - 196. [Abstract] [Full Text] [PDF]

				T. W. R. Lee, G. E. Blair, and D. A. Matthews Adenovirus core protein VII contains distinct sequences that mediate targeting to the nucleus and nucleolus, and colocalization with human chromosomes J. Gen. Virol., December 1, 2003; 84(12): 3423 - 3428. [Abstract] [Full Text] [PDF]

				A. Michienzi, S. Li, J. A. Zaia, and J. J. Rossi A nucleolar TAR decoy inhibitor of HIV-1 replication PNAS, October 29, 2002; 99(22): 14047 - 14052. [Abstract] [Full Text] [PDF]

				H. Chen, T. Wurm, P. Britton, G. Brooks, and J. A. Hiscox Interaction of the Coronavirus Nucleoprotein with Nucleolar Antigens and the Host Cell J. Virol., April 16, 2002; 76(10): 5233 - 5250. [Abstract] [Full Text] [PDF]

				D. A. Matthews Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm J. Virol., January 15, 2001; 75(2): 1031 - 1038. [Abstract] [Full Text]

				W. C. Russell Update on adenovirus and its vectors J. Gen. Virol., November 1, 2000; 81(11): 2573 - 2604. [Full Text]

				L Tarassishin, P Szawlowski, J McLay, A. Kidd, and W. Russell Adenovirus core protein VII displays a linear epitope conserved in a range of human adenoviruses J. Gen. Virol., January 1, 1999; 80(1): 47 - 50. [Abstract]