Journal of General Virology, Vol 79, 1677-1685, Copyright © 1998 by Society for General Microbiology

ARTICLES

Adenovirus core protein V interacts with p32--a protein which is associated with both the mitochondria and the nucleus

DA Matthews and WC Russell
School of Biomedical Sciences, University of St Andrews, UK.

Adenovirus protein V is associated with the DNA-containing virus core and functions as a bridge between the capsid and the core. A yeast two- hybrid analysis performed with a human cDNA library using protein V as 'bait' selected a cellular protein, p32 --described previously as associated with the splicing factor ASF/SF2. By expression and purification of p32 and preparation of an antibody we confirmed the binding of p32 to V by a variety of methods including immune precipitation. We demonstrated that p32 was primarily located in the cytoplasm in association with mitochondria but could also be detected in the nucleus as distinct granules and tubules. By examining infected cells using confocal microscopy and immunofluorescence we were able to follow the intracellular locations of protein V and p32 and it is postulated that p32 is part of a system which imports proteins to the nucleus and that adenovirus hijacks this process to deliver its genome to the nucleus.

This article has been cited by other articles:

				V. Fogal, L. Zhang, S. Krajewski, and E. Ruoslahti Mitochondrial/Cell-Surface Protein p32/gC1qR as a Molecular Target in Tumor Cells and Tumor Stroma Cancer Res., September 1, 2008; 68(17): 7210 - 7218. [Abstract] [Full Text] [PDF]

				C. Ohrmalm and G. Akusjarvi Cellular Splicing and Transcription Regulatory Protein p32 Represses Adenovirus Major Late Transcription and Causes Hyperphosphorylation of RNA Polymerase II. J. Virol., May 1, 2006; 80(10): 5010 - 5020. [Abstract] [Full Text] [PDF]

				R. Berro, K. Kehn, C. de la Fuente, A. Pumfery, R. Adair, J. Wade, A. M. Colberg-Poley, J. Hiscott, and F. Kashanchi Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32. J. Virol., April 1, 2006; 80(7): 3189 - 3204. [Abstract] [Full Text] [PDF]

				Z. Q. Yao, S. N. Waggoner, M. W. Cruise, C. Hall, X. Xie, D. W. Oldach, and Y. S. Hahn SOCS1 and SOCS3 Are Targeted by Hepatitis C Virus Core/gC1qR Ligation To Inhibit T-Cell Function J. Virol., December 15, 2005; 79(24): 15417 - 15429. [Abstract] [Full Text] [PDF]

				M. D. Beatch, J. C. Everitt, L. J. Law, and T. C. Hobman Interactions between Rubella Virus Capsid and Host Protein p32 Are Important for Virus Replication J. Virol., August 15, 2005; 79(16): 10807 - 10820. [Abstract] [Full Text] [PDF]

				X. Liang, Y. C. Shin, R. E. Means, and J. U. Jung Inhibition of Interferon-Mediated Antiviral Activity by Murine Gammaherpesvirus 68 Latency-Associated M2 Protein J. Virol., November 15, 2004; 78(22): 12416 - 12427. [Abstract] [Full Text] [PDF]

				A. L. Eisen-Vandervelde, S. N. Waggoner, Z. Q. Yao, E. M. Cale, C. S. Hahn, and Y. S. Hahn Hepatitis C Virus Core Selectively Suppresses Interleukin-12 Synthesis in Human Macrophages by Interfering with AP-1 Activation J. Biol. Chem., October 15, 2004; 279(42): 43479 - 43486. [Abstract] [Full Text] [PDF]

				C. Chattopadhyay, D. Hawke, R. Kobayashi, and S. N. Maity Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro Nucleic Acids Res., July 8, 2004; 32(12): 3632 - 3641. [Abstract] [Full Text] [PDF]

				Z. Q. Yao, A. Eisen-Vandervelde, S. N. Waggoner, E. M. Cale, and Y. S. Hahn Direct Binding of Hepatitis C Virus Core to gC1qR on CD4+ and CD8+ T Cells Leads to Impaired Activation of Lck and Akt J. Virol., June 15, 2004; 78(12): 6409 - 6419. [Abstract] [Full Text] [PDF]

				T. W. R. Lee, F. J. Lawrence, V. Dauksaite, G. Akusjarvi, G. E. Blair, and D. A. Matthews Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins J. Gen. Virol., January 1, 2004; 85(1): 185 - 196. [Abstract] [Full Text] [PDF]

				S. Laine, A. Thouard, J. Derancourt, M. Kress, D. Sitterlin, and J.-M. Rossignol In Vitro and In Vivo Interactions between the Hepatitis B Virus Protein P22 and the Cellular Protein gC1qR J. Virol., December 1, 2003; 77(23): 12875 - 12880. [Abstract] [Full Text] [PDF]

				K. T. Hall, M. S. Giles, M. A. Calderwood, D. J. Goodwin, D. A. Matthews, and A. Whitehouse The Herpesvirus Saimiri Open Reading Frame 73 Gene Product Interacts with the Cellular Protein p32 J. Virol., October 11, 2002; 76(22): 11612 - 11622. [Abstract] [Full Text] [PDF]

				O. A. Vaughan, M. Alvarez-Reyes, J. M. Bridger, J. L. V. Broers, F. C. S. Ramaekers, M. Wehnert, G. E. Morris, W. G. F. Whitfield, and C. J. Hutchison Both emerin and lamin C depend on lamin A for localization at the nuclear envelope J. Cell Sci., March 9, 2002; 114(14): 2577 - 2590. [Abstract] [Full Text] [PDF]

				M. Robles-Flores, E. Rendon-Huerta, H. Gonzalez-Aguilar, G. Mendoza-Hernandez, S. Islas, V. Mendoza, M. V. Ponce-Castaneda, L. Gonzalez-Mariscal, and F. Lopez-Casillas p32 (gC1qBP) Is a General Protein Kinase C (PKC)-binding Protein. INTERACTION AND CELLULAR LOCALIZATION OF P32-PKC COMPLEXES IN RAT HEPATOCYTES J. Biol. Chem., February 8, 2002; 277(7): 5247 - 5255. [Abstract] [Full Text] [PDF]

				H. Bierne and P. Cossart InlB, a surface protein of Listeria monocytogenes that behaves as an invasin and a growth factor J. Cell Sci., January 9, 2002; 115(17): 3357 - 3367. [Abstract] [Full Text] [PDF]

				M. L. Hayman, M. M. Miller, D. M. Chandler, C. C. Goulah, and L. K. Read The trypanosome homolog of human p32 interacts with RBP16 and stimulates its gRNA binding activity Nucleic Acids Res., December 15, 2001; 29(24): 5216 - 5225. [Abstract] [Full Text] [PDF]

				M. J. Bouchard, L.-H. Wang, and R. J. Schneider Calcium Signaling by HBx Protein in Hepatitis B Virus DNA Replication Science, December 14, 2001; 294(5550): 2376 - 2378. [Abstract] [Full Text] [PDF]

				D. A. Matthews Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm J. Virol., January 15, 2001; 75(2): 1031 - 1038. [Abstract] [Full Text]

				H. C. van Leeuwen and P. O'Hare Retargeting of the mitochondrial protein p32/gC1Qr to a cytoplasmic compartment and the cell surface J. Cell Sci., January 6, 2001; 114(11): 2115 - 2123. [Abstract] [Full Text] [PDF]

				H. E. Bryant, D. A. Matthews, S. Wadd, J. E. Scott, J. Kean, S. Graham, W. C. Russell, and J. B. Clements Interaction between Herpes Simplex Virus Type 1 IE63 Protein and Cellular Protein p32 J. Virol., December 1, 2000; 74(23): 11322 - 11328. [Abstract] [Full Text]

				W. C. Russell Update on adenovirus and its vectors J. Gen. Virol., November 1, 2000; 81(11): 2573 - 2604. [Full Text]

				M. D. Beatch and T. C. Hobman Rubella Virus Capsid Associates with Host Cell Protein p32 and Localizes to Mitochondria J. Virol., June 15, 2000; 74(12): 5569 - 5576. [Abstract] [Full Text]

				J. Jiang, Y. Zhang, A. R. Krainer, and R.-M. Xu Crystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein PNAS, March 30, 1999; 96(7): 3572 - 3577. [Abstract] [Full Text] [PDF]

				P. Storz, A. Hausser, G. Link, J. Dedio, B. Ghebrehiwet, K. Pfizenmaier, and F.-J. Johannes Protein Kinase C {micro} Is Regulated by the Multifunctional Chaperon Protein p32 J. Biol. Chem., August 4, 2000; 275(32): 24601 - 24607. [Abstract] [Full Text] [PDF]

				A. J. Tye, B. Ghebrehiwet, N. Guo, K. N. Sastry, B. K. C. Chow, E. I. B. Peerschke, and B.-L. Lim The Human gC1qR/p32 Gene, C1qBP. GENOMIC ORGANIZATION AND PROMOTER ANALYSIS J. Biol. Chem., May 11, 2001; 276(20): 17069 - 17075. [Abstract] [Full Text] [PDF]

				M. T. Schaerer, K. Kannenberg, P. Hunziker, S. W. Baumann, and E. Sigel Interaction between GABAA Receptor beta Subunits and the Multifunctional Protein gC1q-R J. Biol. Chem., July 6, 2001; 276(28): 26597 - 26604. [Abstract] [Full Text] [PDF]