| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of General Virology, Vol 79, 1725-1734, Copyright © 1998 by Society for General Microbiology
ARTICLES |
W Barclay, Q Li, G Hutchinson, D Moon, A Richardson, N Percy, JW Almond and DJ Evans
School of Animal and Microbial Sciences, University of Reading, Whiteknights, UK.
The inclusion of a foreign marker gene, chloramphenicol acetyltransferase (CAT) gene, into the poliovirus genome allows its replication and encapsidation to be easily monitored using a simple enzyme assay. Such poliovirus replicons require the presence of helper virus for their successful propagation and thus are similar to defective interfering (DI) viruses. In genomes containing the CAT gene, the majority of the P1 virus capsid region of the poliovirus genome could be removed without destroying viability. The smallest replicon was significantly smaller than any naturally occurring DI particle so far reported, yet it retained the ability to replicate and be encapsidated by structural proteins provided by helper virus in trans. The efficiency with which the replicons were encapsidated was investigated using a direct immunostaining technique that allows individual cells infected with either a replicon or helper virus to be quantified. These results were compared to the frequencies of trans- encapsidation of polioviruses and coxsackievirus B4 using a two-stage neutralization assay. Poliovirus types 1, 2 and 3 but not coxsackievirus B4, coxsackievirus A21 or rhinovirus 14 provided efficient trans-encapsidation of poliovirus type 3 or type 3-derived replicons. These results suggest that a specific encapsidation process operates and that it does not involve RNA sequences within the region of the genome encoding the capsid proteins.
This article has been cited by other articles:
|
|
 |
|
  M. Arita, Y. Ami, T. Wakita, and H. Shimizu Cooperative Effect of the Attenuation Determinants Derived from Poliovirus Sabin 1 Strain Is Essential for Attenuation of Enterovirus 71 in the NOD/SCID Mouse Infection Model J. Virol., February 15, 2008; 82(4): 1787 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Arita, N. Nagata, T. Sata, T. Miyamura, and H. Shimizu Quantitative analysis of poliomyelitis-like paralysis in mice induced by a poliovirus replicon. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3317 - 3327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Grubman and B. Baxt Foot-and-Mouth Disease Clin. Microbiol. Rev., April 1, 2004; 17(2): 465 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sasaki and K. Taniguchi The 5'-End Sequence of the Genome of Aichi Virus, a Picornavirus, Contains an Element Critical for Viral RNA Encapsidation J. Virol., March 15, 2003; 77(6): 3542 - 3548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tiley, A. M. Q. King, and G. J. Belsham The Foot-and-Mouth Disease Virus cis-Acting Replication Element (cre) Can Be Complemented in trans within Infected Cells J. Virol., February 1, 2003; 77(3): 2243 - 2246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. G. GOODFELLOW, D. KERRIGAN, and D. J. EVANS Structure and function analysis of the poliovirus cis-acting replication element (CRE) RNA, January 1, 2003; 9(1): 124 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, H.-H. Lu, S. Mueller, and E. Wimmer The C-terminal residues of poliovirus proteinase 2Apro are critical for viral RNA replication but not for cis- or trans-proteolytic cleavage J. Gen. Virol., February 1, 2001; 82(2): 397 - 408. [Abstract] [Full Text]
|
|
|
|
|
|
S. Evans, D. Cavanagh, and P. Britton Utilizing fowlpox virus recombinants to generate defective RNAs of the coronavirus infectious bronchitis virus J. Gen. Virol., December 1, 2000; 81(12): 2855 - 2865. [Abstract] [Full Text]
|
|
|
|
|
|
L. K. Johansen and C. D. Morrow Inherent Instability of Poliovirus Genomes Containing Two Internal Ribosome Entry Site (IRES) Elements Supports a Role for the IRES in Encapsidation J. Virol., September 15, 2000; 74(18): 8335 - 8342. [Abstract] [Full Text]
|
|
|
|
|
|
G. M. McInerney, A. M. Q. King, N. Ross-Smith, and G. J. Belsham Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences J. Gen. Virol., July 1, 2000; 81(7): 1699 - 1702. [Abstract] [Full Text]
|
|
|
|
|
|
I. Goodfellow, Y. Chaudhry, A. Richardson, J. Meredith, J. W. Almond, W. Barclay, and D. J. Evans Identification of a cis-Acting Replication Element within the Poliovirus Coding Region J. Virol., May 15, 2000; 74(10): 4590 - 4600. [Abstract] [Full Text]
|
|
|
|
|
|
R. Molenkamp, B. C. D. Rozier, S. Greve, W. J. M. Spaan, and E. J. Snijder Isolation and Characterization of an Arterivirus Defective Interfering RNA Genome J. Virol., April 1, 2000; 74(7): 3156 - 3165. [Abstract] [Full Text]
|
|
|
|
 |
|
 P.-E. Lobert, N. Escriou, J. Ruelle, and T. Michiels A coding RNA sequence acts as a replication signal in cardioviruses PNAS, September 28, 1999; 96(20): 11560 - 11565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Palese RNA virus vectors: Where are we and where do we need to go? PNAS, October 27, 1998; 95(22): 12750 - 12752. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
