Journal of General Virology, Vol 79, 1775-1780, Copyright © 1998 by Society for General Microbiology

ARTICLES

Sequence analysis of the Hendra virus nucleoprotein gene: comparison with other members of the subfamily Paramyxovirinae

M Yu, E Hansson, B Shiell, W Michalski, BT Eaton and LF Wang
CSIRO Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria.

The nucleoprotein (N) gene of Hendra virus (HeV), an unclassified member of subfamily Paramyxovirinae in the family Paramyxoviridae previously known as equine morbillivirus, was cloned and sequenced. The majority of the deduced amino acid sequence was further confirmed by direct sequencing of peptide fragments of the N protein derived from purified virions. The 3' untranslated sequence of the HeV N gene mRNA was 568 nt and was much longer than that observed in other Paramyxovirinae. The N protein was 532 amino acids in length with a molecular mass of 58.5 kDa. Although the HeV N protein had a slightly higher amino acid sequence identity to those of the genus Morbillivirus than to those of other Paramyxovirinae genera, the level of identity was much lower than that observed within the morbilliviruses. Our results indicated that HeV could not confidently be classified as a member of the genus Morbillivirus, Paramyxovirus or Rubulavirus and suggest that the virus be classified in a new genus within the Paramyxovirinae.

This article has been cited by other articles:

				M. Yoneda, V. Guillaume, F. Ikeda, Y. Sakuma, H. Sato, T. F. Wild, and C. Kai Establishment of a Nipah virus rescue system PNAS, October 31, 2006; 103(44): 16508 - 16513. [Abstract] [Full Text] [PDF]

				P. J. M. Jack, D. B. Boyle, B. T. Eaton, and L.-F. Wang The Complete Genome Sequence of J Virus Reveals a Unique Genome Structure in the Family Paramyxoviridae J. Virol., August 15, 2005; 79(16): 10690 - 10700. [Abstract] [Full Text] [PDF]

				H. Weingartl, S. Czub, J. Copps, Y. Berhane, D. Middleton, P. Marszal, J. Gren, G. Smith, S. Ganske, L. Manning, et al. Invasion of the Central Nervous System in a Porcine Host by Nipah Virus J. Virol., June 15, 2005; 79(12): 7528 - 7534. [Abstract] [Full Text] [PDF]

				Y. P. Chan, C. L. Koh, S. K. Lam, and L.-F. Wang Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of henipaviruses J. Gen. Virol., June 1, 2004; 85(6): 1675 - 1684. [Abstract] [Full Text] [PDF]

				K. Halpin, B. Bankamp, B. H. Harcourt, W. J. Bellini, and P. A. Rota Nipah virus conforms to the rule of six in a minigenome replication assay J. Gen. Virol., March 1, 2004; 85(3): 701 - 707. [Abstract] [Full Text] [PDF]

				L.-F. Wang, M. Yu, E. Hansson, L. I. Pritchard, B. Shiell, W. P. Michalski, and B. T. Eaton The Exceptionally Large Genome of Hendra Virus: Support for Creation of a New Genus within the Family Paramyxoviridae J. Virol., November 1, 2000; 74(21): 9972 - 9979. [Abstract] [Full Text]

				O de Leeuw and B Peeters Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae J. Gen. Virol., January 1, 1999; 80(1): 131 - 136. [Abstract]