J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamada, H.
Right arrow Articles by Nishiyama, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamada, H.
Right arrow Articles by Nishiyama, Y.
Agricola
Right arrow Articles by Yamada, H.
Right arrow Articles by Nishiyama, Y.

Journal of General Virology, Vol 79, 1989-1995, Copyright © 1998 by Society for General Microbiology

ARTICLES

Characterization of the UL55 gene product of herpes simplex virus type 2

H Yamada, YM Jiang, S Oshima, T Daikoku, Y Yamashita, T Tsurumi and Y Nishiyama
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.

We have identified the herpes simplex virus type 2 (HSV-2) UL55 gene product using a rabbit polyclonal antiserum raised against a recombinant 6 x His-UL55 fusion protein expressed in Escherichia coli. The antiserum reacted specifically with a 23 kDa protein in HSV-2 186- infected cell lysates. The protein was not detectable in the presence of the viral DNA synthesis inhibitor phosphonoacetic acid. Indirect immunofluorescence studies localized the UL55 protein within and at the periphery of the nucleus as discrete granules at late times post- infection, and nuclear fractionation studies showed that the protein was associated with the nuclear matrix of infected cells. Moreover, these discrete regions containing the UL55 protein were found to be adjacent to compartments, designated assemblons, containing the capsid protein ICP35. However, the UL55 protein was not detected in purified virions. These results suggest that the UL55 protein of HSV-2 may play an accessory role in virion assembly or maturation.


This article has been cited by other articles:


Home page
 J. Virol.Home page
S. Loret, G. Guay, and R. Lippe
Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
J. Virol., September 1, 2008; 82(17): 8605 - 8618.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Kato, M. Yamamoto, T. Ohno, M. Tanaka, T. Sata, Y. Nishiyama, and Y. Kawaguchi
Herpes Simplex Virus 1-Encoded Protein Kinase UL13 Phosphorylates Viral Us3 Protein Kinase and Regulates Nuclear Localization of Viral Envelopment Factors UL34 and UL31
J. Virol., February 1, 2006; 80(3): 1476 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
Y. Yamauchi, C. Shiba, F. Goshima, A. Nawa, T. Murata, and Y. Nishiyama
Herpes simplex virus type 2 UL34 protein requires UL31 protein for its relocation to the internal nuclear membrane in transfected cells
J. Gen. Virol., June 1, 2001; 82(6): 1423 - 1428.
[Abstract] [Full Text]

Home page
 J. Gen. Virol.Home page
H. Yamada, Y.-M. Jiang, H.-Y. Zhu, K. Inagaki-Ohara, and Y. Nishiyama
Nucleolar localization of the UL3 protein of herpes simplex virus type 2
J. Gen. Virol., August 1, 1999; 80(8): 2157 - 2164.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1998 by the Society for General Microbiology.