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Journal of General Virology, Vol 79, 2157-2161, Copyright © 1998 by Society for General Microbiology

ARTICLES

Transcription-positive cofactor 4 enhances rescue of adeno-associated virus genome from an infectious clone

S Muramatsu, A Handa, S Kajigaya and KE Brown
Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, MD 20892-1652, USA. muramats@nih.gov

While Rep proteins are required for adeno-associated virus (AAV) replication, little is known about cellular proteins that interact with Rep. We demonstrate here that transcription-positive cofactor 4 (PC4, p15) fused to Gal4-activating domain interacted with both AAV-2 and AAV- 3 Rep proteins fused to Gal4 DNA-binding domain, leading to reporter activation in the yeast two-hybrid system. In addition to its coactivating function, PC4 recently has been shown to be involved in replication of simian virus 40. To study a functional role for the PC4- Rep protein interaction, 293-31 cells were cotransfected with a PC4 expression plasmid and an infectious clone of AAV-3, followed by super- infection with helper adenovirus. A significantly increased number of AAV-3 genomes were rescued in PC4 transfected cells. Our results support a possible involvement of PC4 in AAV replication and may be used in efficient production of AAV vectors for gene therapy.


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S. Weger, E. Hammer, and R. Heilbronn
Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
J. Gen. Virol., March 1, 2002; 83(3): 511 - 516.
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