J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 8 (1970), 1-10; DOI 10.1099/0022-1317-8-1-1
© 1970 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oroszlan, S.
Right arrow Articles by Gilden, R. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oroszlan, S.
Right arrow Articles by Gilden, R. V.
Agricola
Right arrow Articles by Oroszlan, S.
Right arrow Articles by Gilden, R. V.

Proteins of the Murine C-Type RNA Tumour Viruses: Isolation of a Group-specific Antigen by Isoelectric Focusing

S. Oroszlan, Carol L. Fisher, T. B. Stanley and R. V. Gilden

Flow Laboratories, Inc., Rockville, Maryland 20852, U.S.A.

The group-specific antigen of two murine C-type RNA tumour virus types was purified to homogeneity by isoelectric-focusing of virus disrupted with tween-ether. The antigen has a molecular weight of 25,000 to 26,000 (estimated from sedimentation in sucrose relative to standards) and an isoelectric point of 6.7. The latter value was obtained from preparations of Friend, Moloney, Rauscher and Gross subgroups. The antigen was immunogenic in guinea-pigs inducing the synthesis of monospecific group-reactive antibodies. The antigen was relatively free of nucleic acid and appeared to be the only virus protein, based on labelling studies, with an isoelectric point near neutrality. Envelope antigen was localized at pH 4.5 by the electrofocusing technique.

Received 14 November 1969; accepted 17 February 1970.


This article has been cited by other articles:


Home page
 ScienceHome page
P. Andersen, M Barbacid, Tronick SR, H. Clark, and S. Aaronson
Evolutionary relatedness of viper and primate endogenous retroviruses
Science, April 20, 1979; 204(4390): 318 - 321.
[Abstract] [PDF]

Home page
 ScienceHome page
S. Oroszlan, T. Copeland, M. R. Summers, and R. V. Gilden
Feline Leukemia and RD-114 Virus Group-Specific Proteins: Comparison of Amino Terminal Sequence
Science, August 3, 1973; 181(4098): 454 - 456.
[Abstract] [PDF]

Home page
 ScienceHome page
S. Oroszlan, D. Bova, R. Toni, and R. V. Gilden
Interactions of Immunoglobulins G and M in the Detection of the Mammalian C-Type Virus Cross-Reactive Antigen
Science, April 28, 1972; 176(4033): 420 - 422.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1970 by the Society for General Microbiology.