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Immunofluorescence Studies of T and Virus Capsid Antigens Induced by Chick Embryo Lethal Orphan Virus

University of Sheffield, Virus Research Laboratory, Lodge Moor Hospital, and Department of Medical Microbiology, The University, Sheffield 10

Chick embryo lethal orphan virus induced both T and virus capsid antigens during cytolytic infection of chick kidney cells. The T antigen was produced relatively early, 8 or 9 hr after infection, while virus capsid antigen was first detected after 15 to 16 hr. The T antigen appeared as a fine granular fluorescence and also as small blobs confined to the cell nucleus. T antigen was detected in virus-infected hamster, mouse and human cells, but was present in very few cells compared with chick kidney monolayers, where almost all cells contained antigen. The virus capsid antigen had a more complex morphology, with fluorescing spots and blobs in the nucleus; it was also detected as a diffuse fluorescence in the cytoplasm later in infection. The production of virus capsid antigen in chick kidney cells was partially inhibited by cytosine arabinoside (100 µg./ml.) or 5-fluoro-2'-deoxyuridine (30 µg./ml.). These inhibitors of DNA viruses had no effect on the production of virus-induced T antigen in chick kidney cells.

No antigenic relationship was detected by immunofluorescence or complement-fixation tests between chick embryo lethal orphan virus and T antigens of adenovirus type 12. In addition, the adenovirus group hexon antigen was not detected in preparations of chick embryo lethal orphan virus by complement-fixation or immunofluorescence tests.

Received 26 January 1970; accepted 5 March 1970.