HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vasconcelos-Costa, J. Search for Related Content PubMed PubMed Citation Articles by Vasconcelos-Costa, J. Agricola Articles by Vasconcelos-Costa, J.

Detection by Immunofluorescence of Surface Antigens in Cells from Tumours Induced in Hamsters by Adenovirus Type 12

Laboratory of Cell Biology Gulbenkian Institute of Science, Oeiras, Portugal

Surface antigens have been detected by immunofluorescence in cells transformed by two oncogenic DNA viruses: polyoma (Irlin, 1967; Malmgren, Takemoto & Carney, 1968) and SV 40 (Tevethia, Katz & Rapp, 1965). This technique provides an easy way to study in vitro surface antigens as virus determinants in tumour cells. Surface antigens, or some of them, are probably tumour-specific transplantation antigens. Adenoviruses, another group of oncogenic DNA viruses, induce tumours whose cells are known to possess tumour specific transplantation antigens (Eddy, Grubbs & Young, 1964; Trentin & Bryan, 1966; Sjögren, Minowada & Ankerst, 1967; Berman, 1967), but surface antigen has not until now been detected by immunofluorescence (Hollinshead & Alford, 1969).

In this work I report the observation by immunofluorescence of surface antigen in cells derived from a hamster tumour induced by adenovirus type 12. A modification of Möller's technique (Möller, 1961) was used, as well as Tevethia's technique with fixed cells (Tevethia et al. 1965).

Received 26 January 1970; accepted 5 March 1970.