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Radiological Studies on the Chronological Relation between Murine Sarcoma Virus Infection and Cell Cycle

Institut du Radium, Biologie, Bâtiment 110, Faculté des Sciences, 91-Orsay, France

Intracellular replication of murine sarcoma virus was followed by Luria-Latarjet experiments. In experiments with unsynchronized cultures ultraviolet sensitivity of the cells infected with murine sarcoma virus as focus centres increased with the lapse of time after infection, and 16 hr after infection u.v.-resistant cells appeared which were probably producing mature virus. The u.v.-resistant population continued to increase thereafter.

Ultraviolet sensitivities as focus centres of synchronized cells infected with murine sarcoma virus at different periods of the cell cycle were compared 23 hr after infection. The cells infected with the virus in the G1 phase remained more sensitive to u.v. light than those infected with the virus in the S phase, i.e. the latent period was longer after G1 phase infection than after S phase infection. An antimitotic agent colchicine, prevented the increase of u.v. resistance of the cells as focus centres. These facts suggest that murine sarcoma virus infection requires the division of the host cells and that virus replication is synchronized at this stage of the cell cycle.

Received 26 January 1970; accepted 30 March 1970.