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Specific Removal of Host-cell or Vaccinia-virus Antigens from Extracts of Infected Cells by Polyvalent Disulphide-linked Immunosorbents

Department of Microbiology, The University of Birmingham, Birmingham 15

Disulphide-linked immunosorbents prepared from rabbit antisera against extracts of uninfected HeLa (ERK) cells specifically removed host-cell antigens from extracts of cells which had been infected with vaccinia virus. Similarly, disulphide-linked immunosorbents prepared from rabbit anti-vaccinia sera specifically removed vaccinia-specific antigens from infected cell extracts. Disulphide-linked preparations derived from unrelated sera adsorbed neither host nor virus-specific antigens.

Received 23 July 1969; accepted 16 April 1970.