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Fate of Adenovirus Types 2 and 12 in Infected Serial Cultures of Non-primate Origin

Department of Microbiology, University of Illinois at the Medical Center, Chicago

In RHF-1 cells infected with either adenovirus 2 or 12, the formation of infectious virus and antigens decreased with each successive passage of cells until the virus was ultimately eliminated from the cultures. These cultures then emerged into a new phase in which some virus-induced proteins were present in at least a small proportion of cells. Adenovirus 2 fibre antigen persisted throughout the 15th subculture, whereas adenovirus 12 early (T) and late (fibre) antigens were carried throughout the 30th subculture over a period of 600 days. Virus-free but antigen-containing cells may therefore have possessed at least a portion of the virus genome. Shortly after the disappearance of virus, distinct multilayered foci of cells emerged in both lines. This phenomenon became a characteristic feature of the cultures only in the adenovirus 12 line.

^* Present address: National Institute for Medical Research, Division of Virology, Mill Hill, London, N.W.7.

Received 9 February 1970; accepted 17 March 1970.