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Laboratoire de Biologie Expérimentale, Faculté des Sciences, Orsay, France
Seventy-one spontaneous thermosensitive mutants of Vesicular Stomatitis Virus were isolated and tested for complementation. The spontaneous mutation rate seems to be very high since 2.3% of mutants were found in wild-type clones. Experiments were performed at 30° (permissive temperature) and 39.8° (non-permissive temperature). The mutants can be classified into five complementation groups. 58 mutants fall into the first group.
Received 10 December 1969;
accepted 7 May 1970.
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