| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of General Virology, Vol 80, 169-177, Copyright © 1999 by Society for General Microbiology
ARTICLES |
Y Wang, R Ling, JC Erker, H Zhang, H Li, S Desai, IK Mushahwar and TJ Harrison
Department of Medicine, Royal Free and University College Medical School of University College London, Royal Free Campus, UK.
Recent studies have reported and provided nucleotide sequence data from divergent isolates of hepatitis E virus (HEV), including isolates from North America and Africa. Sera were investigated from 29 Chinese patients with a diagnosis of acute hepatitis and who were negative for hepatitis viruses A-E by serology (HEV was excluded by testing for IgG antibody only). To determine whether some patients were infected with HEV but had yet to seroconvert to antibody positivity, RT-PCR was carried out with primers designed within conserved sequences of the HEV open reading frame (ORF) 1 and ORF2 regions. Fifteen patients were found to harbour sequences related to HEV. Analysis of the HEV products revealed that nucleotide sequences from nine of the sera closely matched Burmese-like HEV sequences (more than 92% nucleotide identity across ORF1 and 88% in ORF2). The remaining six HEV isolates were similar to each other but divergent from all other known HEV sequences (74 to 83% nucleotide identity in ORF1 or ORF2). Phylogenetic analysis suggests that the six divergent isolates represent a fourth genotype of HEV, distinct from the previously described Burmese, Mexican and United States variants (genotypes 1, 2 and 3). This novel variant, referred to here as the Chinese genotype (genotype 4), may be responsible for a significant proportion of cases of acute hepatitis in China, as seen by the fact that 40% of the HEV-infected patients in this study were genotype 4 positive.
This article has been cited by other articles:
|
|
 |
|
  J. Inoue, M. Takahashi, K. Ito, T. Shimosegawa, and H. Okamoto Analysis of human and swine hepatitis E virus (HEV) isolates of genotype 3 in Japan that are only 81-83 % similar to reported HEV isolates of the same genotype over the entire genome. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2363 - 2369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-m. Ahn, S.-G. Kang, D.-Y. Lee, S. J. Shin, and H. S. Yoo Identification of Novel Human Hepatitis E Virus (HEV) Isolates and Determination of the Seroprevalence of HEV in Korea J. Clin. Microbiol., July 1, 2005; 43(7): 3042 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, S. Kusakai, H. Mizuo, K. Suzuki, K. Fujimura, K. Masuko, Y. Sugai, T. Aikawa, T. Nishizawa, and H. Okamoto Simultaneous Detection of Immunoglobulin A (IgA) and IgM Antibodies against Hepatitis E Virus (HEV) Is Highly Specific for Diagnosis of Acute HEV Infection J. Clin. Microbiol., January 1, 2005; 43(1): 49 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. F. Huang, Z. F. Sun, S. U. Emerson, R. H. Purcell, H. L. Shivaprasad, F. W. Pierson, T. E. Toth, and X. J. Meng Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV J. Gen. Virol., June 1, 2004; 85(6): 1609 - 1618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. F. Sun, C. T. Larsen, A. Dunlop, F. F. Huang, F. W. Pierson, T. E. Toth, and X.-J. Meng Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis-splenomegaly syndrome in different geographical regions of the United States J. Gen. Virol., March 1, 2004; 85(3): 693 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Shrestha, S. Shrestha, F. Tsuda, T. Nishizawa, M. Takahashi, Y. Gotanda, and H. Okamoto Genetic changes in hepatitis E virus of subtype 1a in patients with sporadic acute hepatitis E in Kathmandu, Nepal, from 1997 to 2002 J. Gen. Virol., January 1, 2004; 85(1): 97 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yazaki, H. Mizuo, M. Takahashi, T. Nishizawa, N. Sasaki, Y. Gotanda, and H. Okamoto Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food J. Gen. Virol., September 1, 2003; 84(9): 2351 - 2357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-S. Choi, H.-J. Kwon, N.-R. Shin, and H. S. Yoo Identification of Swine Hepatitis E Virus (HEV) and Prevalence of Anti-HEV Antibodies in Swine and Human Populations in Korea J. Clin. Microbiol., August 1, 2003; 41(8): 3602 - 3608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nishizawa, M. Takahashi, H. Mizuo, H. Miyajima, Y. Gotanda, and H. Okamoto Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome J. Gen. Virol., May 1, 2003; 84(5): 1245 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, T. Nishizawa, H. Miyajima, Y. Gotanda, T. Iita, F. Tsuda, and H. Okamoto Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus J. Gen. Virol., April 1, 2003; 84(4): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tokita, H. Harada, Y. Gotanda, M. Takahashi, T. Nishizawa, and H. Okamoto Molecular and serological characterization of sporadic acute hepatitis E in a Japanese patient infected with a genotype III hepatitis E virus in 1993 J. Gen. Virol., January 1, 2003; 84(2): 421 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. F. Huang, G. Haqshenas, H. L. Shivaprasad, D. K. Guenette, P. R. Woolcock, C. T. Larsen, F. W. Pierson, F. Elvinger, T. E. Toth, and X. J. Meng Heterogeneity and Seroprevalence of a Newly Identified Avian Hepatitis E Virus from Chickens in the United States J. Clin. Microbiol., November 1, 2002; 40(11): 4197 - 4202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mizuo, K. Suzuki, Y. Takikawa, Y. Sugai, H. Tokita, Y. Akahane, K. Itoh, Y. Gotanda, M. Takahashi, T. Nishizawa, et al. Polyphyletic Strains of Hepatitis E Virus Are Responsible for Sporadic Cases of Acute Hepatitis in Japan J. Clin. Microbiol., September 1, 2002; 40(9): 3209 - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Haqshenas, F. F. Huang, M. Fenaux, D. K. Guenette, F. W. Pierson, C. T. Larsen, H. L. Shivaprasad, T. E. Toth, and X. J. Meng The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virus J. Gen. Virol., September 1, 2002; 83(9): 2201 - 2209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, T. Nishizawa, A. Yoshikawa, S. Sato, N. Isoda, K. Ido, K. Sugano, and H. Okamoto Identification of two distinct genotypes of hepatitis E virus in a Japanese patient with acute hepatitis who had not travelled abroad J. Gen. Virol., August 1, 2002; 83(8): 1931 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. F. Huang, G. Haqshenas, D. K. Guenette, P. G. Halbur, S. K. Schommer, F. W. Pierson, T.E. Toth, and X. J. Meng Detection by Reverse Transcription-PCR and Genetic Characterization of Field Isolates of Swine Hepatitis E Virus from Pigs in Different Geographic Regions of the United States J. Clin. Microbiol., April 1, 2002; 40(4): 1326 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. J. Meng, B. Wiseman, F. Elvinger, D. K. Guenette, T. E. Toth, R. E. Engle, S. U. Emerson, and R. H. Purcell Prevalence of Antibodies to Hepatitis E Virus in Veterinarians Working with Swine and in Normal Blood Donors in the United States and Other Countries J. Clin. Microbiol., January 1, 2002; 40(1): 117 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, H. Zhang, Z. Li, W. Gu, H. Lan, W. Hao, R. Ling, H. Li, and T. J. Harrison Detection of Sporadic Cases of Hepatitis E Virus (HEV) Infection in China Using Immunoassays Based on Recombinant Open Reading Frame 2 and 3 Polypeptides from HEV Genotype 4 J. Clin. Microbiol., December 1, 2001; 39(12): 4370 - 4379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Haqshenas, H. L. Shivaprasad, P. R. Woolcock, D. H. Read, and X. J. Meng Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States J. Gen. Virol., October 1, 2001; 82(10): 2449 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. E. Williams, C. Kasorndorkbua, P. G. Halbur, G. Haqshenas, D. K. Guenette, T. E. Toth, and X. J. Meng Evidence of Extrahepatic Sites of Replication of the Hepatitis E Virus in a Swine Model J. Clin. Microbiol., September 1, 2001; 39(9): 3040 - 3046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kabrane-Lazizi, M. Zhang, R. H. Purcell, K. D. Miller, R. T. Davey, and S. U. Emerson Acute hepatitis caused by a novel strain of hepatitis E virus most closely related to United States strains J. Gen. Virol., July 1, 2001; 82(7): 1687 - 1693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Halbur, C. Kasorndorkbua, C. Gilbert, D. Guenette, M. B. Potters, R. H. Purcell, S. U. Emerson, T. E. Toth, and X. J. Meng Comparative Pathogenesis of Infection of Pigs with Hepatitis E Viruses Recovered from a Pig and a Human J. Clin. Microbiol., March 1, 2001; 39(3): 918 - 923. [Abstract] [Full Text]
|
|
|
|
|
|
H. C. Worm, G. G. Schlauder, H. Wurzer, and I. K. Mushahwar Identification of a novel variant of hepatitis E virus in Austria: sequence, phylogenetic and serological analysis J. Gen. Virol., December 1, 2000; 81(12): 2885 - 2890. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Wang, H. Zhang, R. Ling, H. Li, and T. J. Harrison The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3 J. Gen. Virol., July 1, 2000; 81(7): 1675 - 1686. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Buisson, M. Grandadam, E. Nicand, P. Cheval, H. van Cuyck-Gandre, B. Innis, P. Rehel, P. Coursaget, R. Teyssou, and S. Tsarev Identification of a novel hepatitis E virus in Nigeria J. Gen. Virol., April 1, 2000; 81(4): 903 - 909. [Abstract] [Full Text]
|
|
|
|
|
|
S.-Y. Hsieh, X.-J. Meng, Y.-H. Wu, S.-T. Liu, A. W. Tam, D.-Y. Lin, and Y.-F. Liaw Identity of a Novel Swine Hepatitis E Virus in Taiwan Forming a Monophyletic Group with Taiwan Isolates of Human Hepatitis E Virus J. Clin. Microbiol., December 1, 1999; 37(12): 3828 - 3834. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
