J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alloul, N.
Right arrow Articles by Sherman, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alloul, N.
Right arrow Articles by Sherman, L.
Agricola
Right arrow Articles by Alloul, N.
Right arrow Articles by Sherman, L.

Journal of General Virology, Vol 80, 29-37, Copyright © 1999 by Society for General Microbiology

ARTICLES

The E2 protein of human papillomavirus type 16 is translated from a variety of differentially spliced polycistronic mRNAs

N Alloul and L Sherman
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.

The major regulation protein of human papillomavirus (HPV) transcription is the viral E2 protein. Previous studies have identified a variety of alternatively spliced mRNAs containing multiple open reading frames (ORFs) encoding the E2 protein of HPV type 16. In these mRNAs the E2 ORF is contained as an internal ORF. In the present study, the translational capacities of three mRNA species starting at the p97 promoter and containing the 880/2581, 880/2708 and 226/2708 splice junctions upstream of the E2 ORF were investigated. Partial cDNAs spanning the E2 ORF and the related upstream ORFs were synthesized and assessed for E2 protein translation in vivo, in COS cells, and in vitro, in cell-free systems. Results of these analyses indicated that E2 protein was translated from all three mRNAs. Translation efficiency of E2 from the natural polycistronic templates was lower compared with that from a synthetic monocistronic control. Translation from the d- type bicistronic template (226/2708) was more efficient than that from the a-type (880/2708) and a'-type (880/2561) polycistronic templates. Further investigation of the translation of proteins encoded by the ORFs preceding the E2 ORF showed that a- and a'-type templates served for translation mainly of E7 but also of E61, while the d-type template served for translation of E6IV. Overall, the translation data support the suggestion that the corresponding mRNAs may function as polycistronic transcripts.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
R. M. Ordonez, A. M. Espinosa, D. J. Sanchez-Gonzalez, J. Armendariz-Borunda, and J. Berumen
Enhanced oncogenicity of Asian-American human papillomavirus 16 is associated with impaired E2 repression of E6/E7 oncogene transcription
J. Gen. Virol., June 1, 2004; 85(6): 1433 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
W. Deng, G. Jin, B.-Y. Lin, B. A. Van Tine, T. R. Broker, and L. T. Chow
mRNA Splicing Regulates Human Papillomavirus Type 11 E1 Protein Production and DNA Replication
J. Virol., October 1, 2003; 77(19): 10213 - 10226.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
N. Alloul and L. Sherman
Transcription-modulatory activities of differentially spliced cDNAs encoding the E2 protein of human papillomavirus type 16
J. Gen. Virol., September 1, 1999; 80(9): 2461 - 2470.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1999 by the Society for General Microbiology.