HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gao, Y. Articles by Splitter, G. A. Search for Related Content PubMed PubMed Citation Articles by Gao, Y. Articles by Splitter, G. A. Agricola Articles by Gao, Y. Articles by Splitter, G. A.

Mapping T and B lymphocyte epitopes of bovine herpesvirus-1 glycoprotein B

Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, Madison, WI 53706, USA¹

Author for correspondence: Gary Splitter.Fax +1 608 262 7420. e-mail splitter{at}ahabs.wisc.edu

Glycoprotein B (gB) is a major envelope protein of bovine herpesvirus-1 (BHV-1). As a subunit vaccine, the extracellular domain of recombinant gB induces neutralizing antibody and T cell responses that engender protection against virus challenge. Here, lymphocytes from animals of different parentage were analysed for T cell proliferation to the gB extracellular domain for immune recognition. Four truncated overlapping gB gene segments encoding 742 amino acids were expressed from a baculovirus vector to identify antigenic regions. One immunodominant region (amino acids 254–532) was recognized by T cells from immune individuals of different parentage. Serial synthetic peptides spanning this region localized the T cell (amino acids 319–340 and 415–436) and B cell (amino acids 331–352, 475–496 and 487–508) epitopes. Elucidation of gB epitopes indicates the diverse and distinctive recognition by T cells and antibodies of this envelope glycoprotein by cattle, the natural host of BHV-1.

This article has been cited by other articles:

				L. BenMohamed, G. Bertrand, C. D. McNamara, H. Gras-Masse, J. Hammer, S. L. Wechsler, and A. B. Nesburn Identification of Novel Immunodominant CD4+ Th1-Type T-Cell Peptide Epitopes from Herpes Simplex Virus Glycoprotein D That Confer Protective Immunity J. Virol., September 1, 2003; 77(17): 9463 - 9473. [Abstract] [Full Text] [PDF]

				S. S. Coberley, R. C. Condit, L. H. Herbst, and P. A. Klein Identification and Expression of Immunogenic Proteins of a Disease-Associated Marine Turtle Herpesvirus J. Virol., September 11, 2002; 76(20): 10553 - 10558. [Abstract] [Full Text] [PDF]