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Temporal mapping of transcripts in human herpesvirus-7

Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via L. Borsari 46, 44100 Ferrara , Italy¹

Author for correspondence: Dario Di Luca.Fax +39 0532 247618. e-mail dil{at}dns.unife.it

Transcription of human herpesvirus-7 (HHV-7) in cultures of productively infected T-cells was studied. Transcription of HHV-7 was regulated by the typical herpesvirus cascade in which , ß and genes are sequentially transcribed. Transcripts of U10, U14, U18, U31, U39, U41, U42, U53, U73 and U89/90 were detected 3 h after infection and were not inhibited by the absence of protein synthesis and therefore were functions. U19 and U18/20 were ß genes; their transcription was inhibited by cycloheximide but not by phosphonoacetate, an inhibitor of DNA synthesis. U60/66 and U98/100 were genes since their spliced transcripts were not detected in cells treated with phosphonoacetate. HHV-7 transcription was regulated by complex mechanisms, which involve the temporal coordinated activation of specific viral promoters and post-transcriptional processing. Splice mechanisms were also temporally regulated. Transcription of U89/90 pre-mRNA and splice took place simultaneously in the immediate-early phase. On the other hand, U16/17 pre-mRNA was synthesized with typical kinetics, but the spliced product was regulated as a ß function. Likewise, the primary transcripts of U60/66 and U98/100 were and ß, respectively, but both spliced products were synthesized in the late phase of virus replication. Finally, HHV-7 supported a bona fide latent infection in the adult population, since viral transcripts were not detected in peripheral blood mononuclear cells of healthy donors infected with HHV-7.

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