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Journal of General Virology (1999), 80, 2809-2812.
© 1999 Society for General Microbiology

Plant

Effect of mutations within the Cys-rich region of potyvirus helper component-proteinase on self-interaction

Silvio Urcuqui-Inchima1, Ivan G. Maia2, Gabrièle Drugeon 1, Anne-Lise Haenni1 and Françoise Bernardi1

Institut Jacques Monod, 2 Place Jussieu – Tour 43, 75251 Paris Cedex 05, France1
Centro de Biologia Molecular e Engenharia Genética, UNICAMP, CP 6109, Campinas – SP, Brazil2

Author for correspondence: Silvio Urcuqui-Inchima.Fax +33 1 44 27 35 80. e- mail urcuqui{at}ijm.jussieu.fr (On leave from the Centro Fruticola Andino, Cali, Colombia.)

The first ~60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment.




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