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Journal of General Virology (1999), 80, 2861-2865.
© 1999 Society for General Microbiology

Animal: DNA Viruses

Free thiol groups are essential for infectivity of human cytomegalovirus

Ali Mirazimi1, Mehrdad Mousavi-Jazi1, Vivi-Anne Sundqvist2 and Lennart Svensson1

Department of Virology, SMI/Karolinska Institute, 171 82 Solna, Sweden 1
Department of Immunology, Microbiology, Pathology and Infectious Disease, Karolinska Institute, Box 12 773, 112 96 Stockholm, Sweden 2

Author for correspondence: Lennart Svensson.Fax +46 8 301635. e-mail Lensve{at}mbox.ki.se

The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.




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