| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: DNA Viruses |
Department of Medical Virology1 and Department of Medicine3, University of Tü bingen, Calwerstraße 7/6, D-72076 Tübingen, Germany
Department of Pathology, Ohio State University, Columbus, USA 2
Author for correspondence: Christian Sinzger.Fax +49 7071 295790.e-mail christian.sinzger{at}med.uni-tuebingen.de
Following extensive propagation in fibroblasts, human cytomegalovirus (HCMV) loses tropism for a number of otherwise natural host cells, in particular, endothelial cells. In this study, the hypothesis was tested that loss of endothelial tropism is associated with the appearance of genomic variants. Initial quantitative focus expansion assays on endothelial monolayers demonstrated that, while the laboratory strains AD169 and Towne failed to form detectable foci, 29 out of 30 recent clinical HCMV isolates had the potential to expand in endothelial cell culture. By long-term adaptation in fibroblast cultures, nonendotheliotropic strains could be selected from clinical HCMV isolates, while long-term endothelial-adapted strains of the same isolates retained both fibroblast tropism and endothelial tropism. Such differentially adapted isolate pairs always displayed genomic differences in restriction fragment length analyses. Coinfection of endothelial cells by two nonendotheliotropic HCMV strains yielded an endotheliotropic recombinant HCMV variant combining portions of the genomes of both parental viruses. When DNA purified from various isolates was transfected into fibroblasts, progeny virus retained the specific tropism of parental virus from which the DNA was isolated. These findings demonstrate that endothelial tropism is an inherent property of most clinical HCMV isolates and is determined by the viral genome. Although the specific determinants of HCMV cell tropism are still unknown, this study provides the first evidence for a genetic contribution.
This article has been cited by other articles:
|
|
 |
|
  A. L. Vanarsdall, B. J. Ryckman, M. C. Chase, and D. C. Johnson Human Cytomegalovirus Glycoproteins gB and gH/gL Mediate Epithelial Cell-Cell Fusion When Expressed either in cis or in trans J. Virol., December 1, 2008; 82(23): 11837 - 11850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schuessler, K. L. Sampaio, and C. Sinzger Charge Cluster-to-Alanine Scanning of UL128 for Fine Tuning of the Endothelial Cell Tropism of Human Cytomegalovirus J. Virol., November 15, 2008; 82(22): 11239 - 11246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. T. K. Le, M. Trilling, M. Wilborn, H. Hengel, and A. Zimmermann Human cytomegalovirus interferes with signal transducer and activator of transcription (STAT) 2 protein stability and tyrosine phosphorylation J. Gen. Virol., October 1, 2008; 89(10): 2416 - 2426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kessler, M. Reich, G. Jahn, E. Tolosa, A. Beck, H. Kalbacher, H. Overkleeft, S. Schempp, and C. Driessen Human cytomegalovirus infection interferes with major histocompatibility complex type II maturation and endocytic proteases in dendritic cells at multiple levels J. Gen. Virol., October 1, 2008; 89(10): 2427 - 2436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. J. Jiang, B. Adler, K. L. Sampaio, M. Digel, G. Jahn, N. Ettischer, Y.-D. Stierhof, L. Scrivano, U. Koszinowski, M. Mach, et al. UL74 of Human Cytomegalovirus Contributes to Virus Release by Promoting Secondary Envelopment of Virions J. Virol., March 15, 2008; 82(6): 2802 - 2812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sinzger, G. Hahn, M. Digel, R. Katona, K. L. Sampaio, M. Messerle, H. Hengel, U. Koszinowski, W. Brune, and B. Adler Cloning and sequencing of a highly productive, endotheliotropic virus strain derived from human cytomegalovirus TB40/E J. Gen. Virol., February 1, 2008; 89(2): 359 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Ryckman, B. L. Rainish, M. C. Chase, J. A. Borton, J. A. Nelson, M. A. Jarvis, and D. C. Johnson Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial Cells J. Virol., January 1, 2008; 82(1): 60 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cicin-Sain, I. Bubic, M. Schnee, Z. Ruzsics, C. Mohr, S. Jonjic, and U. H. Koszinowski Targeted Deletion of Regions Rich in Immune-Evasive Genes from the Cytomegalovirus Genome as a Novel Vaccine Strategy J. Virol., December 15, 2007; 81(24): 13825 - 13834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Stern-Ginossar, N. Elefant, A. Zimmermann, D. G. Wolf, N. Saleh, M. Biton, E. Horwitz, Z. Prokocimer, M. Prichard, G. Hahn, et al. Host Immune System Gene Targeting by a Viral miRNA Science, July 20, 2007; 317(5836): 376 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Jarvis and J. A. Nelson Human Cytomegalovirus Tropism for Endothelial Cells: Not All Endothelial Cells Are Created Equal J. Virol., March 1, 2007; 81(5): 2095 - 2101. [Full Text] [PDF]
|
|
|
|
|
|
B. Adler, L. Scrivano, Z. Ruzcics, B. Rupp, C. Sinzger, and U. Koszinowski Role of human cytomegalovirus UL131A in cell type-specific virus entry and release J. Gen. Virol., September 1, 2006; 87(9): 2451 - 2460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. Kvale, J. Dalgaard, F. Lund-Johansen, H. Rollag, L. Farkas, K. Midtvedt, F. L. Jahnsen, J. E. Brinchmann, and J. Olweus CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection Blood, March 1, 2006; 107(5): 2022 - 2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang and T. Shenk Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism PNAS, December 13, 2005; 102(50): 18153 - 18158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Spaderna, B. Kropff, Y. Kodel, S. Shen, S. Coley, S. Lu, W. Britt, and M. Mach Deletion of gpUL132, a Structural Component of Human Cytomegalovirus, Results in Impaired Virus Replication in Fibroblasts J. Virol., September 15, 2005; 79(18): 11837 - 11847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang and T. Shenk Human Cytomegalovirus UL131 Open Reading Frame Is Required for Epithelial Cell Tropism J. Virol., August 15, 2005; 79(16): 10330 - 10338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Patrone, M. Secchi, L. Fiorina, M. Ierardi, G. Milanesi, and A. Gallina Human Cytomegalovirus UL130 Protein Promotes Endothelial Cell Infection through a Producer Cell Modification of the Virion J. Virol., July 1, 2005; 79(13): 8361 - 8373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Reinhardt, T. Mertens, U. Mayr-Beyrle, H. Frank, A. Luske, K. Schierling, and J. Waltenberger HCMV infection of human vascular smooth muscle cells leads to enhanced expression of functionally intact PDGF {beta}-receptor Cardiovasc Res, July 1, 2005; 67(1): 151 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Carlson, W. L. W. Chang, S. S. Zhou, A. F. Tarantal, and P. A. Barry Rhesus brain microvascular endothelial cells are permissive for rhesus cytomegalovirus infection J. Gen. Virol., March 1, 2005; 86(3): 545 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Sampaio, Y. Cavignac, Y.-D. Stierhof, and C. Sinzger Human Cytomegalovirus Labeled with Green Fluorescent Protein for Live Analysis of Intracellular Particle Movements J. Virol., March 1, 2005; 79(5): 2754 - 2767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rahbar and C. Soderberg-Naucler Human Cytomegalovirus Infection of Endothelial Cells Triggers Platelet Adhesion and Aggregation J. Virol., February 15, 2005; 79(4): 2211 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schierling, T. Stamminger, T. Mertens, and M. Winkler Human Cytomegalovirus Tegument Proteins ppUL82 (pp71) and ppUL35 Interact and Cooperatively Activate the Major Immediate-Early Enhancer J. Virol., September 1, 2004; 78(17): 9512 - 9523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.H. Shen, B. Utama, J. Wang, M. Raveendran, D. Senthil, W.J. Waldman, J.D. Belcher, G. Vercellotti, D. Martin, B.M. Mitchelle, et al. Human Cytomegalovirus Causes Endothelial Injury Through the Ataxia Telangiectasia Mutant and p53 DNA Damage Signaling Pathways Circ. Res., May 28, 2004; 94(10): 1310 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dolan, C. Cunningham, R. D. Hector, A. F. Hassan-Walker, L. Lee, C. Addison, D. J. Dargan, D. J. McGeoch, D. Gatherer, V. C. Emery, et al. Genetic content of wild-type human cytomegalovirus J. Gen. Virol., May 1, 2004; 85(5): 1301 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamamoto-Tabata, S. McDonagh, H.-T. Chang, S. Fisher, and L. Pereira Human Cytomegalovirus Interleukin-10 Downregulates Metalloproteinase Activity and Impairs Endothelial Cell Migration and Placental Cytotrophoblast Invasiveness In Vitro J. Virol., March 15, 2004; 78(6): 2831 - 2840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Murphy, D. Yu, J. Grimwood, J. Schmutz, M. Dickson, M. A. Jarvis, G. Hahn, J. A. Nelson, R. M. Myers, and T. E. Shenk Coding potential of laboratory and clinical strains of human cytomegalovirus PNAS, December 9, 2003; 100(25): 14976 - 14981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tarrago, C. Quereda, and A. Tenorio Different Cytomegalovirus Glycoprotein B Genotype Distribution in Serum and Cerebrospinal Fluid Specimens Determined by a Novel Multiplex Nested PCR J. Clin. Microbiol., July 1, 2003; 41(7): 2872 - 2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Akter, C. Cunningham, B. P. McSharry, A. Dolan, C. Addison, D. J. Dargan, A. F. Hassan-Walker, V. C. Emery, P. D. Griffiths, G. W. G. Wilkinson, et al. Two novel spliced genes in human cytomegalovirus J. Gen. Virol., May 1, 2003; 84(5): 1117 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Minisini, C. Tulone, A. Luske, D. Michel, T. Mertens, P. Gierschik, and B. Moepps Constitutive Inositol Phosphate Formation in Cytomegalovirus-Infected Human Fibroblasts Is due to Expression of the Chemokine Receptor Homologue pUS28 J. Virol., April 15, 2003; 77(8): 4489 - 4501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ellsmore, G. G. Reid, and N. D. Stow Detection of human cytomegalovirus DNA replication in non-permissive Vero and 293 cells J. Gen. Virol., March 1, 2003; 84(3): 639 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Maisch, B. Kropff, C. Sinzger, and M. Mach Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus J. Virol., November 13, 2002; 76(24): 12803 - 12812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Spaderna, H. Blessing, E. Bogner, W. Britt, and M. Mach Identification of Glycoprotein gpTRL10 as a Structural Component of Human Cytomegalovirus J. Virol., February 1, 2002; 76(3): 1450 - 1460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pignatelli, P. Dal Monte, and M. P. Landini gpUL73 (gN) genomic variants of human cytomegalovirus isolates are clustered into four distinct genotypes J. Gen. Virol., November 1, 2001; 82(11): 2777 - 2784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Grazia Revello, F. Baldanti, E. Percivalle, A. Sarasini, L. De-Giuli, E. Genini, D. Lilleri, N. Labò, and G. Gerna In vitro selection of human cytomegalovirus variants unable to transfer virus and virus products from infected cells to polymorphonuclear leukocytes and to grow in endothelial cells J. Gen. Virol., June 1, 2001; 82(6): 1429 - 1438. [Abstract] [Full Text]
|
|
|
|
|
|
B. P. McSharry, C. J. Jones, J. W. Skinner, D. Kipling, and G. W. G. Wilkinson Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus J. Gen. Virol., April 1, 2001; 82(4): 855 - 863. [Abstract] [Full Text]
|
|
|
|
|
|
C. Sinzger, M. Kahl, K. Laib, K. Klingel, P. Rieger, B. Plachter, and G. Jahn Tropism of human cytomegalovirus for endothelial cells is determined by a post-entry step dependent on efficient translocation to the nucleus J. Gen. Virol., December 1, 2000; 81(12): 3021 - 3035. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kahl, D. Siegel-Axel, S. Stenglein, G. Jahn, and C. Sinzger Efficient Lytic Infection of Human Arterial Endothelial Cells by Human Cytomegalovirus Strains J. Virol., August 15, 2000; 74(16): 7628 - 7635. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
