HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, X. Articles by Ebbesen, P. Search for Related Content PubMed PubMed Citation Articles by Liu, X. Articles by Ebbesen, P. Agricola Articles by Liu, X. Articles by Ebbesen, P.

Transcriptional activation of human TR3/nur77 gene expression by human T-lymphotropic virus type I Tax protein through two AP-1-like elements

Department of Virus and Cancer, Danish Cancer Society, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark¹
George H. Whipple Laboratory for Cancer Research, Departments of Pathology, Urology and Biochemistry, University of Rochester, Box 626, Rochester, NY 14642, USA²

Author for correspondence: Xiangdong Liu.Fax +45 86 19 54 15. e-mail liu{at}virus.au.dk

The Tax transactivator of human T-lymphotropic virus type I (HTLV-I) is capable of inducing expression of the human immediate-early TR3/nur77 gene. Deletion and mutation analyses of the TR3/nur77 promoter demonstrated that multiple transcription elements in the 121 bp sequence proximal to the transcription start site are required for full Tax transactivation. Mutations of CArG-like, Ets and RCE motifs in this region severely decreased Tax transactivation. Mutation of either of the two identical AP-1-like elements (NAP 1 and 2) immediately upstream of the TATA box caused around 80% reduction of Tax transactivation. Mutation of both NAP elements blocked Tax-mediated activation totally. These two NAP elements could confer Tax-responsiveness on a heterologous basal promoter. Furthermore, the specific NAP-binding complex was only observed in HTLV-I-infected cells. Formation of this specific NAP-binding complex was correlated directly with Tax expression, as demonstrated in JPX-9 cells upon induction of Tax expression. The specific NAP binding could be competed for by consensus AP-1 and CREB elements, indicating that the NAP-binding proteins probably belong to the AP-1 and CREB/ATF transcription factor families. Supershift analysis with antibodies to both the AP-1 and CREB/ATF transcription factor families revealed that only anti-JunD antibody could partially shift this NAP-binding complex, indicating that JunD is a component of the NAP complex. This work suggests that JunD is involved in Tax-regulated TR3/nur77 expression.

This article has been cited by other articles:

				G P Taylor Molecular aspects of HTLV-I infection and adult T-cell leukaemia/lymphoma J. Clin. Pathol., December 1, 2007; 60(12): 1392 - 1396. [Abstract] [Full Text] [PDF]

				J. Martinez-Gonzalez and L. Badimon The NR4A subfamily of nuclear receptors: new early genes regulated by growth factors in vascular cells Cardiovasc Res, February 15, 2005; 65(3): 609 - 618. [Abstract] [Full Text] [PDF]

				K. Waldele, G. Schneider, T. Ruckes, and R. Grassmann Interleukin-13 Overexpression by Tax Transactivation: a Potential Autocrine Stimulus in Human T-Cell Leukemia Virus-Infected Lymphocytes J. Virol., June 15, 2004; 78(12): 6081 - 6090. [Abstract] [Full Text] [PDF]

				J. W. Tullai, M. E. Schaffer, S. Mullenbrock, S. Kasif, and G. M. Cooper Identification of Transcription Factor Binding Sites Upstream of Human Genes Regulated by the Phosphatidylinositol 3-Kinase and MEK/ERK Signaling Pathways J. Biol. Chem., May 7, 2004; 279(19): 20167 - 20177. [Abstract] [Full Text] [PDF]

				J.-W. Choi, S. C. Park, G. H. Kang, J. O. Liu, and H.-D. Youn Nur77 Activated by Hypoxia-Inducible Factor-1{alpha} Overproduces Proopiomelanocortin in von Hippel-Lindau-Mutated Renal Cell Carcinoma Cancer Res., January 1, 2004; 64(1): 35 - 39. [Abstract] [Full Text] [PDF]

				L. Chen, S. Ma, B. Li, T. Fink, V. Zachar, M. Takahashi, J. Cuttichia, L.-C. Tsui, P. Ebbesen, and X. Liu Transcriptional activation of immediate-early gene ETR101 by human T-cell leukaemia virus type I Tax J. Gen. Virol., December 1, 2003; 84(12): 3203 - 3214. [Abstract] [Full Text] [PDF]

				J. Basbous, C. Arpin, G. Gaudray, M. Piechaczyk, C. Devaux, and J.-M. Mesnard The HBZ Factor of Human T-cell Leukemia Virus Type I Dimerizes with Transcription Factors JunB and c-Jun and Modulates Their Transcriptional Activity J. Biol. Chem., October 31, 2003; 278(44): 43620 - 43627. [Abstract] [Full Text] [PDF]

				S. O. Kim, K. Ono, P. S. Tobias, and J. Han Orphan Nuclear Receptor Nur77 Is Involved in Caspase-independent Macrophage Cell Death J. Exp. Med., June 2, 2003; 197(11): 1441 - 1452. [Abstract] [Full Text] [PDF]

				K.-H. Song, K. Lee, and H.-S. Choi Endocrine Disrupter Bisphenol A Induces Orphan Nuclear Receptor Nur77 Gene Expression and Steroidogenesis in Mouse Testicular Leydig Cells Endocrinology, June 1, 2002; 143(6): 2208 - 2215. [Abstract] [Full Text] [PDF]

				K.-H. Song, J.-I. Park, M.-O. Lee, J. Soh, K. Lee, and H.-S. Choi LH Induces Orphan Nuclear Receptor Nur77 Gene Expression in Testicular Leydig Cells Endocrinology, December 1, 2001; 142(12): 5116 - 5123. [Abstract] [Full Text] [PDF]