| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Departments of Haematology1 and Medicine2, University of Cambridge, School of Clinical Medicine, Cambridge, UK
Author for correspondence: Juraj Petrik. Present address: Edinburgh and South East Scotland Blood Transfusion Service, Royal Infirmary, Lauriston Place, Edinburgh EH3 9HB, UK.Fax +44 131 5365352.
The unique poly(U/UC) tract, the middle part of the tripartite 3' non-coding region (3'NCR) of hepatitis C virus (HCV) genomic RNA, may represent a recognition signal for the HCV replicase complex. In this study, several proteins binding specifically to immobilized ribooligonucleotide r(U)25 mimicking this structure were identified using cytosolic extracts from HCV-negative or -positive liver explants, and a prominent 36 kDa protein was studied further. Competition experiments including homoribopolymers revealed binding affinities in the order: oligo/poly(U)>>(A)>>(C)>>(G). The protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a multifunctional protein known to bind RNA. GAPDH bound efficiently to the full-length HCV RNA and binding to various 3'NCR constructs revealed critical dependence upon the presence of the middle part of the 3'NCR. Polypyrimidine tract-binding protein, described previously to bind the 3'NCR, did not bind efficiently to the middle part of 3'NCR and was captured from liver extracts in considerably smaller quantities.
This article has been cited by other articles:
|
|
 |
|
  Y. Zhou, X. Yi, J. B. Stoffer, N. Bonafe, M. Gilmore-Hebert, J. McAlpine, and S. K. Chambers The Multifunctional Protein Glyceraldehyde-3-Phosphate Dehydrogenase Is Both Regulated and Controls Colony-Stimulating Factor-1 Messenger RNA Stability in Ovarian Cancer Mol. Cancer Res., August 1, 2008; 6(8): 1375 - 1384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. You and C. M. Rice 3' RNA Elements in Hepatitis C Virus Replication: Kissing Partners and Long Poly(U) J. Virol., January 1, 2008; 82(1): 184 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Randall, M. Panis, J. D. Cooper, T. L. Tellinghuisen, K. E. Sukhodolets, S. Pfeffer, M. Landthaler, P. Landgraf, S. Kan, B. D. Lindenbach, et al. Cellular cofactors affecting hepatitis C virus infection and replication PNAS, July 31, 2007; 104(31): 12884 - 12889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Harris, Z. Zhang, B. Chaubey, and V. N. Pandey Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome Mol. Cell. Proteomics, June 1, 2006; 5(6): 1006 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Graff, H. Nguyen, C. Kasorndorkbua, P. G. Halbur, M. St. Claire, R. H. Purcell, and S. U. Emerson In Vitro and In Vivo Mutational Analysis of the 3'-Terminal Regions of Hepatitis E Virus Genomes and Replicons J. Virol., January 15, 2005; 79(2): 1017 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. El-Hage and G. Luo Replication of hepatitis C virus RNA occurs in a membrane-bound replication complex containing nonstructural viral proteins and RNA J. Gen. Virol., October 1, 2003; 84(10): 2761 - 2769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Stuyver, T. R. McBrayer, P. M. Tharnish, A. E. A. Hassan, C. K. Chu, K. W. Pankiewicz, K. A. Watanabe, R. F. Schinazi, and M. J. Otto Dynamics of Subgenomic Hepatitis C Virus Replicon RNA Levels in Huh-7 Cells after Exposure to Nucleoside Antimetabolites J. Virol., October 1, 2003; 77(19): 10689 - 10694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Evguenieva-Hackenberg, E. Schiltz, and G. Klug Dehydrogenases from All Three Domains of Life Cleave RNA J. Biol. Chem., November 22, 2002; 277(48): 46145 - 46150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Nanda and J. L. Leibowitz Mitochondrial Aconitase Binds to the 3' Untranslated Region of the Mouse Hepatitis Virus Genome J. Virol., April 1, 2001; 75(7): 3352 - 3362. [Abstract] [Full Text]
|
|
|
|
|
|
T. Pietschmann, V. Lohmann, G. Rutter, K. Kurpanek, and R. Bartenschlager Characterization of Cell Lines Carrying Self-Replicating Hepatitis C Virus RNAs J. Virol., February 1, 2001; 75(3): 1252 - 1264. [Abstract] [Full Text]
|
|
|
|
|
|
J. Wood, R. M. Frederickson, S. Fields, and A. H. Patel Hepatitis C Virus 3'X Region Interacts with Human Ribosomal Proteins J. Virol., February 1, 2001; 75(3): 1348 - 1358. [Abstract] [Full Text]
|
|
|
|
|
|
M. Yi, D. E. Schultz, and S. M. Lemon Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function J. Virol., July 15, 2000; 74(14): 6459 - 6468. [Abstract] [Full Text]
|
|
|
|
|
|
R. Bartenschlager and V. Lohmann Replication of hepatitis C virus J. Gen. Virol., July 1, 2000; 81(7): 1631 - 1648. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
